• Ampicillin sodium packaged and labeled.

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SKU: A042

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Description

Ampicillin sodium is a member of the β-lactam family structurally similar to penicillin. The compound inhibits bacterial cell wall synthesis.  Ampicillin resistance is used as a selectable marker to confirm successful cell transformation, as only cells containing plasmid-encoded ESBLs (Extended Spectrum B-lactamases) survive.   Ampicillin is freely soluble in water (50 mg/ml).

TOKU-E offers five forms of Ampicillin:

    CAS Number

    69-52-3

    Molecular Formula

    C16H18N3NaO4S

    Molecular Weight

    371.39

    Mechanism of Action

    Like all β-lactams, Ampicillin interferes with PBP (penicillin binding protein) activity otherwise involved in the final phase of peptidoglycan synthesis. PBP’s are enzymes which catalyze a pentaglycine crosslink between alanine and lysine residues. Without a pentaglycine crosslink, the integrity of the cell wall is severely compromised ultimately leading to cell lysis.

    Storage Conditions

    2-8°C

    Tariff Code

    2941.10.1000

    Spectrum

    Ampicillin sodium targets Gram-negative non ESBL (Extended Spectrum β-lactamase) bacteria including Staphylococcus and Streptococcus species and medically important enteric pathogens such as Shigella and Salmonella. Interestingly, ampicillin has been found to be effective against certain β-lactam sensitive VRE or vancomycin resistant Enterococcus; a glycopeptide antibiotic resistant "superbug."

Applications

    Eukaryotic Cell Culture Applications

    Ampicillin is routinely used to select for cells containing the pcDNA3.1 and pEAK10 resistance plasmids in cell line A904L at an effective concentration of 50 µg/mL. For additional information on your cell culture needs, please visit our cell-culture database.

    Microbiology Applications

    Ampicillin sodium is often used to select for cells that have been transformed with a plasmid containing the ampR gene which confers resistance to ampicillin. Ampicillin sodium is typically used at a concentration of 50-100 µg/mL.

    Media Supplements

    Ampicillin sodium can be used as a selective agent in several types of isolation media:

    Aeromonas Medium Base - Ampicillin Selective Supplement

Specifications

    Form

    Powder

    Appearance

    White or almost white powder, hygroscopic

    Source

    Semi-synthetic

    Water Content (Karl Fischer)

    ≤2.0%

    pH

    8.0-10.0

    Assay

    (anhydrous basis): 845-988 µg/mg

    Impurities

    Dimethylanilline: Meets the requirements
    Methylene Chloride: ≤0.2%

References

    References

    Pitout JD, Sanders CC, Sanders WE (1997) Antimicrobial resistance with focus on beta-lactam resistance in gram-negative bacilli. Am. J. Med 103(1):51-59 PMID 9236486

    Waxman DJ and Strominger JL (1983) Penicillin-binding proteins and the mechanism of action of beta-lactam antibiotics. Ann. Rev. Biochem 52:825-869 PMID 6351730

    Yang W, Zhang L, Lu Z, Tao W, Zhai Z (2001) A new method for protein coexpression in Escherichia coliusing two incompatible plasmids. Protein. Expr. Purif. 22(3):472-478 PMID 11483011

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