SKU: A009  / 
    CAS Number: 7177-48-2

    Ampicillin Trihydrate, USP

    $99.00 - $210.00

    Ampicillin Trihydrate, USP is a member of the β-lactam family similar in structure to penicillin. Ampicillin Trihydrate is slightly soluble in water (10 mg/mL) and soluble in 1 N HCl (50 mg/mL). 

    We also offer:

    • Ampicillin/Sulbactam (2:1) (A071)
    • Ampicillin Anhydrous (A043)
    • Ampicillin Sodium (A042)
    • Ampicillin Trihydrate, EP (A020)

    Ampicillin Trihydrate, USP conforms to United States Pharmacopoeia specifications.

    Mechanism of Action Like all β-lactams, Ampicillin interferes with PBP (penicillin binding protein) activity otherwise involved in the final phase of peptidoglycan synthesis. PBP’s are enzymes which catalyze a pentaglycine crosslink between alanine and lysine residues. Without a pentaglycine crosslink, the integrity of the cell wall is severely compromised ultimately leading to cell lysis.
    Spectrum Ampicillin, USP targets Gram-negative non-ESBL (Extended Spectrum β-lactamase) bacteria including Staphylococcus and Streptococcus species and medically important enteric pathogens such as Shigella and Salmonella. Interestingly, ampicillin has been found to be effective against certain β-lactam sensitive VRE or vancomycin resistant Enterococcus; a glycopeptide antibiotic resistant "superbug." Resistance to Ampicillin is routinely utilized as a selectable marker to confirm successful cell transformation.
    Microbiology Applications Ampicillin Trihydrate is often used to select for cells that have been transformed with a plasmid containing the ampR gene which confers resistance to Ampicillin.

    Media Supplements

    Ampicillin Trihydrate can be used as a selective agent in several types of isolation media:

    Aeromonas Medium Base - Ampicillin Selective Supplement

    Eukaryotic Cell Culture Applications Ampicillin is routinely used to select for cells containing the pcDNA3.1 and pEAK10 resistance plasmids in cell line A904L at an effective concentration of 50 µg/mL. For additional information, please visit our cell-culture database.
    Molecular Formula C16H19N3O4S·3H2O
    Solubility Slightly soluble in water (10 mg/mL) and soluble in 1 N HCl (50 mg/mL)

    Pitout JD, Sanders CC, Sanders WE (1997) Antimicrobial resistance with focus on beta-lactam resistance in gram-negative bacilli. Am. J. Med 103(1):51-59 PMID 9236486

    Waxman DJ and Strominger JL (1983) Penicillin-binding proteins and the mechanism of action of beta-lactam antibiotics. Ann. Rev. Biochem 52:825-869 PMID 6351730

    Yang W, Zhang L, Lu Z, Tao W, Zhai Z (2001) A new method for protein coexpression in Escherichia coli using two incompatible plasmids. Protein. Expr. Purif. 22(3):472-478 PMID 11483011