SKU: A043  / 
    CAS Number: 69-53-4

    Ampicillin Dried

    $76.03 - $496.26

    Ampicillin Anhydrous is a member of the ë_-lactam family and is similar in structure to penicillin.  

    TOKU-E offers five forms of Ampicillin:

    Ampicillin Anhydrous is the most stable and pure form that TOKU-E offers.   It is sparingly soluble in water and freely soluble in 1 N NH4OH (50 mg/mL). 

    Mechanism of ActionLike all β-lactams, Ampicillin interferes with PBP (penicillin binding protein) activity otherwise involved in the final phase of peptidoglycan synthesis. PBP’s are enzymes which catalyze a pentaglycine crosslink between alanine and lysine residues. Without a pentaglycine crosslink, the integrity of the cell wall is severely compromised ultimately leading to cell lysis.
    SpectrumAmpicillin targets Gram-negative, non ESBL (Extended Spectrum β-lactamase) bacteria including Staphylococcus and Streptococcus and medically important enteric pathogens such as Shigella and Salmonella. Interestingly, ampicillin has been found to be effective against certain β-lactam sensitive VRE or vancomycin resistant Enterococcus; a glycopeptide antibiotic resistant "superbug."
    Microbiology ApplicationsAmpicillin Anhydrous is often used to select for cells that have been transformed with a plasmid containing the ampR gene which confers resistance to Ampicillin. Ampicillin is typically used at a concentration of 50-100 µg/mL.

    Media Supplements

    Ampicillin Anhydrous can be used as a selective agent in several types of isolation media:

    Aeromonas Medium Base - Ampicililn Selective Supplement

    Eukaryotic Cell Culture ApplicationsAmpicillin is routinely used to select for cells containing the pcDNA3.1 and pEAK10 resistance plasmids in cell line A904L at an effective concentration of 50 µg/mL. For additional information on your cell culture needs, please visit our cell-culture database.
    Molecular FormulaC16H19N3O4S
    Solubilitysparingly soluble in water and freely soluble in 1 N NH4OH (50 mg/mL).
    References

    Pitout JD, Sanders CC, Sanders WE (1997) Antimicrobial resistance with focus on beta-lactam resistance in gram-negative bacilli. Am. J. Med 103(1):51-59 PMID 9236486

    Waxman DJ and Strominger JL (1983) Penicillin-binding proteins and the mechanism of action of beta-lactam antibiotics. Ann. Rev. Biochem 52:825-869 PMID 6351730

    Yang W, Zhang L, Lu Z, Tao W, Zhai Z (2001) A new method for protein coexpression in Escherichia coliusing two incompatible plasmids. Protein. Expr. Purif. 22(3):472-478 PMID 11483011

    MICDiplococcus pneumoniae| 0.01 - 0.04| 1413| Haemophilus influenzae| 0.04 - 0.8| 1413| Neisseria gonorrhoeae (penicillin-resistant)| 4 - 128| 1408| Neisseria gonorrhoeae (penicillin-susceptible)| 0.008 - 0.25| 1408| Stenotrophomonas maltophilia| >32 - ?| 938|