Ampicillin trihydrate, USP packaged and labeled.

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  • Description

    Ampicillin trihydrate, USP is a member of the extended spectrum β-lactam family and similar in structure to penicillin. Ampicillin resistance is frequently due to secretion of plasmid encoded ESBLs.

    TOKU-E offers five forms of ampicillin:

    In aqueous solution, ampicillin sodium is freely soluble (50 mg/mL). Ampicillin trihydrate is slightly soluble in water (10 mg/mL) and freely soluble in 1 N HCl (50 mg/mL). Ampicillin anhydrous is sparingly soluble in water and freely soluble in 1 N NH4OH (50 mg/mL). Ampicillin sodium is commonly used to select for successfully transformed bacteria. Ampicillin anhydrous (powder) is the most stable and pure form of ampicillin TOKU-E offers.

    Mechanism of Action

    Like all β-lactams, ampicillin interferes with PBP (penicillin binding protein) activity otherwise involved in the final phase of peptidoglycan synthesis. PBP’s are enzymes which catalyze a pentaglycine crosslink between alanine and lysine residues. Without a pentaglycine crosslink, the integrity of the cell wall is severely compromised ultimately leading to cell lysis.

    Spectrum

    Ampicillin, USP targets non-ESBL (Extended Spectrum β-lactamase) bacteria including Staphylococcus and Streptococcus species and medically important enteric pathogens such as Shigella and Salmonella. Interestingly, ampicillin has been found to be effective against certain β-lactam sensitive VRE or vancomycin resistant Enterococcus; a glycopeptide antibiotic resistant "superbug." Resistance to ampicillin is routinely utilized as a selectable marker to confirm successful cell transformation.

    • Microbiology Applications

      Ampicillin trihydrate is often used to select for cells that have been transformed with a plasmid containing the ampR gene which confers resistance to ampicillin. Ampicillin sodium is typically used at a concentration of 50-100 µg/mL and 35-50 µg/mL.

      Media Supplements

      Ampicillin trihydrate can be used as a selective agent in several types of isolation media:

      Aeromonas Medium Base - Ampicililn Selective Supplement

    • Eukaryotic Cell Culture Applications

      Ampicillin is routinely used to select for cells containing the pcDNA3.1 and pEAK10 resistance plasmids in cell line A904L at an effective concentration of 50 µg/mL. For additional information on your cell culture needs, please visit our cell-culture database.

    • References

      Guzmán, Flavio, MD. "Beta Lactams Antibiotics (penicillins and Cephalosporins) Mechanism of Action.” Medical Pharmacology. Pharmacology Corner, 29 Nov. 2008. Web. 21 Aug. 2012.

      Pitout JD, Sanders CC, Sanders WE Jr. Antimicrobial resistance with focus on beta-lactam resistance in gram-negative bacilli. Am J Med 1997; 103:51.

    Form

    Powder

    Tariff Code

    2941.10.1000

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    For in vitro research use only. Not suitable for human or animal consumption. For MSDSs not available online, please email a request to info@toku-e.com

  • CAS Number7177-48-2
    Molecular FormulaC16H19N3O4S·3H2O
    Molecular Weight403.44
    FormPowder
    AppearanceWhite crystalline powder
    SourceSemi-synthetic
    Water Content (Karl Fisher)12.0%-15.0%
    pH3.5 - 6.0
    Storage Conditions≤30°C
    Assay(On Dried Basis): 900-1050µg/mg
    ImpuritiesDimethylaniline: Meets the specifications
    IdentificationIR - Passes test
  • Documents