SKU: A235-20ML  / 
    CAS Number: 69-52-3

    Ampicillin Sodium ReadyMade™ Aqueous Solution


    Ampicillin Sodium ReadyMadeTM Aqueous Solution is provided as a sterile-filtered solution of Ampicillin Sodium formulated in water at a concentration of 100 mg/ml. It has been filter-sterilized using a 0.22 μm filter.

    Ampicillin Sodium is a member of the β-lactam family structurally similar to penicillin. It has an amino group side chain attached to the penicillin structure, 6-amino-penicillanic acid. Ampicillin resistance is used as a selectable marker to confirm successful cell transformation, as only cells containing plasmid-encoded ESBLs (Extended Spectrum B-lactamases) survive.

    We also offer:

    • Ampicillin Sodium (A042)
    Mechanism of Action Like all β-lactams, Ampicillin interferes with PBP (penicillin binding protein) activity otherwise involved in the final phase of peptidoglycan synthesis. PBPs are enzymes which catalyze a pentaglycine crosslink between alanine and lysine residues. Without a pentaglycine crosslink, the integrity of the cell wall is severely compromised ultimately leading to cell lysis.
    Spectrum Ampicillin is broad-spectrum, effective against both Gram-positive and Gram-negative bacteria including Gram-negative non-ESBL (Extended Spectrum β-lactamase) bacteria including Staphylococcus and Streptococcus species and medically important enteric pathogens such as Shigella and Salmonella. Interestingly, Ampicillin has been found to be effective against certain β-lactam sensitive VRE or vancomycin resistant Enterococcus; a glycopeptide antibiotic resistant "superbug." Resistance to Ampicillin is routinely used as a selectable marker to confirm successful cell transformation.
    Microbiology Applications

    Ampicillin Sodium is often used to select for cells that have been transformed with a plasmid containing the bla gene which confers resistance to Ampicillin. Ampicillin Sodium is typically used at a concentration of 50-100 µg/mL. It can also be used with pBR322 and pUC derivatives.

    Media Supplements
    Ampicillin Sodium can be used as a selective agent in several types of isolation media:

    Aeromonas Medium Base - Ampicillin Selective Supplement

    Eukaryotic Cell Culture Applications Ampicillin is routinely used to select for cells containing the pcDNA3.1 and pEAK10 resistance plasmids in cell line A904L at an effective concentration of 50 µg/mL.  For additional information, please visit our Cell-Culture Database.
    Molecular Formula C16H18N3NaO4

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