Ampicillin sodium packaged and labeled.

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  • Description

    Ampicillin sodium is a member of the extended spectrum β-lactam family and similar in structure to penicillin. Resistance Ampicillin resistance is due to cells containing plasmid encoded ESBLs. 

    TOKU-E offers five forms of ampicillin:

    In aqueous solution, ampicillin sodium is freely soluble (50 mg/mL). Ampicillin trihydrate is slightly soluble in water (10 mg/mL) and freely soluble in 1 N HCl (50 mg/mL). Ampicillin anhydrous is sparingly soluble in water and freely soluble in 1 N NH4OH (50 mg/mL). Ampicillin sodium is commonly used to select for successfully transformed bacteria. Ampicillin anhydrous (powder) is the most stable and pure form of ampicillin TOKU-E offers.

    Mechanism of Action

    Like all β-lactams, ampicillin interferes with PBP (penicillin binding protein) activity otherwise involved in the final phase of peptidoglycan synthesis. PBP’s are enzymes which catalyze a pentaglycine crosslink between alanine and lysine residues. Without a pentaglycine crosslink, the integrity of the cell wall is severely compromised ultimately leading to cell lysis.

    Spectrum

    Ampicillin sodium targets non ESBL (Extended Spectrum β-lactamase) bacteria including Staphylococcus and Streptococcus species and medically important enteric pathogens such as Shigella and Salmonella. Interestingly, ampicillin has been found to be effective against certain β-lactam sensitive VRE or vancomycin resistant Enterococcus; a glycopeptide antibiotic resistant "superbug." Resistance to ampicillin is routinely utilized as a selection antibiotic to confirm successful cell transformation.

    • Microbiology Applications

      Ampicillin sodium is often used to select for cells that have been transformed with a plasmid containing the ampR gene which confers resistance to ampicillin. Ampicillin sodium is typically used at a concentration of 50-100 µg/mL.

      Media Supplements

      Ampicillin sodium can be used as a selective agent in several types of isolation media:

      Aeromonas Medium Base - Ampicililn Selective Supplement

    • Eukaryotic Cell Culture Applications

      Ampicillin is routinely used to select for cells containing the pcDNA3.1 and pEAK10 resistance plasmids in cell line A904L at an effective concentration of 50 µg/mL. For additional information on your cell culture needs, please visit our cell-culture database.

    • References

      Guzmán, Flavio, MD. "Beta Lactams Antibiotics (penicillins and Cephalosporins) Mechanism of Action.” Medical Pharmacology. Pharmacology Corner, 29 Nov. 2008. Web. 21 Aug. 2012.

      Pitout JD, Sanders CC, Sanders WE Jr. Antimicrobial resistance with focus on beta-lactam resistance in gram-negative bacilli. Am J Med 1997; 103:51.

    Form

    Powder

    Tariff Code

    2941.10.1000

    Related Documents


    For in vitro research use only. Not suitable for human or animal consumption. For MSDSs not available online, please email a request to [email protected]

  • CAS Number69-52-3
    Molecular FormulaC16H18N3NaO4S
    Molecular Weight371.39
    FormPowder
    AppearanceWhite or almost white powder, hygroscopic
    SourceSemi-synthetic
    Water Content (Karl Fisher)≤2.0%
    pH8.0-10.0
    Storage Conditions2-8°C
    Assay(anhydrous basis): 845-988 µg/mg
    ImpuritiesDimethylanilline: Meets the requirements
    Methylene Chloride: ≤0.2%
    IdentificationPasses test
  • Documents