SKU: A020

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Description

Ampicillin Trihydrate is a member of the β-lactam family and is similar in structure to penicillin.

TOKU-E offers five forms of Ampicillin:


Ampicillin Trihydrate is slightly soluble in water (10 mg/mL) and freely soluble in 1 N HCl (50 mg/mL). 

    CAS Number

    7177-48-2

    Molecular Formula

    C16H19N3O4S·3H2O

    Molecular Weight

    403.44

    Mechanism of Action

    Like all β-lactams, Ampicillin Trihydrate interferes with PBP (penicillin binding protein) activity otherwise involved in the final phase of peptidoglycan synthesis. PBP’s are enzymes which catalyze a pentaglycine crosslink between alanine and lysine residues. Without a pentaglycine crosslink, the integrity of the cell wall is severely compromised ultimately leading to cell lysis.

    Storage Conditions

    ≤30°C

    Tariff Code

    2941.10.1000

    Spectrum

    Ampicillin Trihydrate, EP targets non-ESBL (Extended Spectrum β-lactamase) bacteria including Staphylococcus and Streptococcus species and medically important enteric pathogens such as Shigella and Salmonella. Interestingly, ampicillin has been found to be effective against certain β-lactam sensitive VRE or vancomycin resistant Enterococcus; a glycopeptide antibiotic resistant "superbug." Resistance to ampicillin is routinely utilized as a selectable marker to confirm successful cell transformation.

Applications

    Eukaryotic Cell Culture Applications

    Ampicillin is routinely used to select for cells containing the pcDNA3.1 and pEAK10 resistance plasmids in cell line A904L at an effective concentration of 50 µg/mL. For additional information on your cell culture needs, please visit our cell-culture database.

    Microbiology Applications

    Ampicillin Trihydrate is often used to select for cells that have been transformed with a plasmid containing the ampR gene which confers resistance to Ampicillin.

    Media Supplements

    Ampicillin trihydrate can be used as a selective agent in several types of isolation media:

    Aeromonas Medium Base - Ampicillin Selective Supplement

Specifications

    Appearance

    White crystalline powder

    Source

    Semi-synthetic

    Water Content (Karl Fischer)

    12.0%-15.0%

    pH

    3.5 -5.5

    Optical Rotation

    +280° to +305°

    Assay

    (On Dried Basis): 96.0-100.5%

    Sulfated Ash

    ≤0.5%

    Impurities

    Dimethylaniline: ≤20ppm
    Related Substances: ≤1.0%

References

    References

    Pitout JD, Sanders CC, Sanders WE (1997) Antimicrobial resistance with focus on beta-lactam resistance in gram-negative bacilli. Am. J. Med 103(1):51-59 PMID 9236486

    Waxman DJ and Strominger JL (1983) Penicillin-binding proteins and the mechanism of action of beta-lactam antibiotics. Ann. Rev. Biochem 52:825-869 PMID 6351730

    Yang W, Zhang L, Lu Z, Tao W, Zhai Z (2001) A new method for protein coexpression in Escherichia coli using two incompatible plasmids. Protein. Expr. Purif. 22(3):472-478 PMID 11483011

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