Application
Cycloheximide is used in molecular biology for ribosome profiling / translational profiling to understand the complexity of the initiation of translation. Cycloheximide can also be used to determine the half-life of proteins, and select for CHX-resistant strains of yeast/fungi.
Cancer Applications
Pretreatment with cycloheximide followed by estrogen stimulation prevented the estrogen-induced changes in glucose metabolism in perfused breast cancer T47D clone 11 cells. This suggested that the estrogen stimulation requires synthesis of mRNA and protein (Neeman and Degani, 1989).
In studying the “immune escape” of cancer cells, in human colorectal cancer cell line COLO 205 is normally resistant to TNF-alpha - a death inducing ligand. However, co-incubation TNF-alpha with cycloheximide caused time-dependent cell death. In fact, authors found that Cycloheximide sensitizes cells to TNF-alpha-induced apoptosis (Pajak et al, 2005).
Electrophoresis Applications
Cycloheximide is widely used in biomedical research to inhibit protein synthesis in eukaryotic cells studied in vitro. It inhibits the synthesis of proteins and macromolecules,and affects apoptosis in eukaryotes.
Microbiology Applications
Media Supplements
Cycoloheximide is routinely used as a selection agent in several types of isolation media:
Columbia Blood Agar - Campylobacter selective supplement (Butzler)
Dermasel agar - Selective supplement for dermatophyte fungi
Campylobacter Agar - Campylobacter Selective Supplement (Preston)
Listeria Selective Agar - Listeria Selective Supplement
Listeria Enrichemnt Broth - Listeria Selective Enrichment Supplement
Listeria Enrichment Broth - Modified Listeria Selective Enrichemnt Supplement
STAA Agar - STAA Selective Supplement
Legionella CYE Agar - Legionella GVPC Selective Supplement
Campylobacter Agar - Campylobacter Selective Supplement (Karmali)
Bolton Broth - Bolton Broth Selective Supplement
Plant Biology Applications
Cycloheximide is a commonly used lab reagent used in in vitro applications to inhibit fungal growth by targeting protein synthesis. In yeast, concentrations of 200 uM have fungicidal effects (Schneider-Poetsch et al, 2009).
The compound can be used as a plant growth regulator to stimulate ethylene production in leaves and fruit.