• Cycloheximide A, EvoPure packaged and labeled.

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SKU: C123

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Description

Cycloheximide A, EvoPure® is a highly purified (≥99.0) form of Cycloheximide A, the active compound in standard grade cycloheximide.  Cycloheximide is a glutarimide antibiotic and natural fungicide isolated from Streptomyces griseus and a protein synthesis inhibitor in eukaryotic cells.  It was discovered by Alma Whiffen-Barksdale of Upjohn Company in 1946.  It is routinely used as a selection agent in several types of isolation media.  It can be used as a tool in molecular biology to determine the half life of proteins, or in in chase experiments to analyze protein degradation. 

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    CAS Number

    66-81-9

    Molecular Formula

    C15H23NO4

    Molecular Weight

    281.35

    Mechanism of Action

    Cycloheximide binds to the ribosome and inhibits the eEF2-mediated translocation step in protein synthesis, thus blocking translational elongation.

    Storage Conditions

    -20°C

    Tariff Code

    2941.90.1050

    Spectrum

    Cycloheximide is used for fungi and yeast, including fungi found in brewing test media. It has lower activity against bacteria.

Applications

    Application

    Cycloheximide is used in molecular biology for ribosome profiling / translational profiling to understand the complexity of translation initiation. Cycloheximide is used to study protein synthesis, expression and degradation, and determine the half-life of proteins.

    Cancer Applications

    Pretreatment with cycloheximide followed by estrogen stimulation prevented the estrogen-induced changes in glucose metabolism in perfused breast cancer T47D clone 11 cells. This suggested that the estrogen stimulation requires synthesis of mRNA and protein (Neeman and Degani, 1989). In studying the “immune escape” of cancer cells, in human colorectal cancer cell line COLO 205 is normally resistant to TNF-alpha - a death inducing ligand. However, co-incubation TNF-alpha with cycloheximide caused time-dependent cell death. In fact, authors found that Cycloheximide sensitizes cells to TNF-alpha-induced apoptosis (Pajak et al, 2005).

    Eukaryotic Cell Culture Applications

    Cycloheximide is widely used in biomedical research to inhibit protein synthesis in eukaryotic cells studied in vitro. It inhibits the synthesis of proteins and macromolecules,and affects apoptosis in eukaryotes.

    Microbiology Applications

    Cycloheximide chase followed by western blotting was used to analyze protein degradation in the model unicellular eukaryote, S. cerevisiae (buddiing yeast). Yeast cells are incubated in cycloheximide and cell aliquots are collected after specific time points. This allows visualization of the degradation kinetics of the steady state population of a variety of cellular proteins (Buchanan et al, 2016).

    Representative susceptibility data includes:

    • Candida albicans: 12.5 µg/ml
    • Saccharomyces cerevisiae: 0.2 µg/ml
    • Mycosphaerella graminicola: 5.62-100 µg/ml

     For additional Cycloheximide MIC data, please review our Antimicrobial Index.

    Plant Biology Applications

    Cycloheximide is a commonly used lab reagent used in in vitro applications to inhibit fungal growth by targeting protein synthesis. In yeast, concentrations of 200 uM have fungicidal effects (Schneider-Poetsch et al, 2009). The compound can be used as a plant growth regulator to stimulate ethylene production in leaves and fruit.

Specifications

    Form

    Powder

    Appearance

    White or cream-colored powder

    Source

    Streptomyces griseus

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