• Cycloheximide packaged and labeled.

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SKU: C001

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Cycloheximide is a protein synthesis inhibitor in eukaryotic cells and is commonly used to prevent fungal growth. 

TOKU-E offers three forms of cycloheximide: cycloheximide (C001), cycloheximide solution (C084), and cycloheximide ultrapure (C071). Cycloheximide solution is dissolved in DMSO at 100 mg/mL. Cycloheximide ultrapure is ≥98% pure and is free of toxic isomers that can potentially kill wanted fungi in a selection media.

This product is considered a dangerous good. Quantities above 1 g may be subject to additional shipping fees. Please contact us for specific questions.

Kadir et al. used cycloheximide from TOKU-E in culture medium to prevent de-novo translation of DVL proteins in neuroblastoma SH-SY5Y cells. "ALFY-Controlled DVL3 Autophagy Regulates Wnt Signaling, Determining Human Brain Size"  

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    Mechanism of Action

    Cycloheximide prevents ribosomal translocation during translation as shown in a yeast model. Effective doses are present at concentrations around 200 µM to inhibit yeast growth.

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    Microbiology Applications

    Media Supplements

    Cycoloheximide is routinely used as a selection agent in several types of isolation media:

    Columbia Blood AgarCampylobacter selective supplement (Butzler)

    Dermasel agar - Selective supplement for dermatophyte fungi

    Campylobacter Agar - Campylobacter Selective Supplement (Preston)

    Listeria Selective Agar - Listeria Selective Supplement

    Listeria Enrichemnt Broth - Listeria Selective Enrichment Supplement

    Listeria Enrichment Broth - Modified Listeria Selective Enrichemnt Supplement

    STAA Agar - STAA Selective Supplement

    Legionella CYE Agar - Legionella GVPC Selective Supplement

    Campylobacter Agar - Campylobacter Selective Supplement (Karmali)

    Bolton Broth - Bolton Broth Selective Supplement

    Plant Biology Applications

    Cycloheximide is a frequently used lab reagent to inhibit fungal growth by targeting protein synthesis. In yeast, concentrations of 200 µM have fungicidal effects.





    White or cream-colored powder


    Streptomyces griseus

    Potency (on a dry basis)

    ≥900 u/mg (on dried basis)

    Loss on Drying

    ≤ 1.0%



    Doyle S.M., Diamond M. and McCabe P.F, 2009, Chloroplast and reactive oxygen species involvement in apoptotic-like programmed cell death in Arabidopsis suspension cultures. Journal of Experimental Botany, Vol. 61, No. 2, pp. 473–482, 2010

    Schneider-Poetsch T., Ju J., Eyler D.E., Dang Y., Bhat S., Merrick W.C., Green R. and Shen B., 2009, Inhibition of eukaryotic translation elongation by cycloheximide and lactimidomycin. Nature chemical biology, vol 6, march 2010.

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