SKU: O004  / 
    CAS Number: 2058-46-0

    Oxytetracycline Hydrochloride

    S/.228.42 - S/.899.10

    Oxytetracycline Hydrochloride is the hydrochloride salt of Oxytetracycline, the second tetracycline antibiotic to be discovered.  Oxytetracycline is a broad-spectrum tetracycline that was discovered near the Pfizer laboratories in a soil sample containing Streptomyces rimosus by Finlay et al., in 1950. Together with the chemist Robert B Woodward, they deciphered the chemical structure which allowed Pfizer to produce it commercially under the trade name Terramycin. Oxytetracycline can be used for studying the oxytetracycline-resistance gene (otrA).

    Oxytetracycline Hydrochloride is soluble in water.

    We also offer:

    • Oxytetracycline Dihydrate (O003)

     

    Mechanism of Action The mechanism of Oxytetracycline involves diffusing through a cell and binding to the 30s ribosomal subunit preventing peptide elongation and ultimately inhibiting protein synthesis. Resistance to Oxytetracycline can be a result of inactivation by cell enzymes or pumping the antibiotic out of the cell upon entering.
    Spectrum Oxytetracycline is a broad-spectrum antibiotic which targets Gram-positive and Gram-negative species as well as a few Mycoplasma species
    Microbiology Applications

    Oxytetracycline is commonly used in clinical in vitro microbiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against Gram-positive, Gram-negative, and Mycoplasma microbial isolates. Medical microbiologists use AST results to recommend antibiotic treatment options. 

    Representative MIC values include:

    • Haemophilus influenzae 1.6 µg/mL – 6.3 µg/mL
    • Mycoplasma bovis 0.12 µg/mL - 4 µg/mL
    • For a representative list of Oxytetracycline MIC values, click here.

    Media Supplement

    Oxytetracycline is used as a selective agent in OGYE, a selective media for yeasts and molds.

    Plant Biology Applications Oxytetracycline has been used to prevent fire blight in apple and pears in the Pacific Northwest region of the USA. In a study by Hubbard et al.,  Oxytetracycline was shown to be a better alternative to streptomycin to inhibit fire blight disease.
    Molecular Formula C22H24N2O9•HCl
    Solubility Ethanol (95%) 33 mg/mL
    Ethanol (absolute): 12 mg/mL
    Water: slightly soluble
    Note: Concentrated aqueous solutions at neutral pH hydrolyze and precipitates crystals of oxytetracycline
    References

    Chopra I and Roberts M (2001)  Tetracycline Antibiotics: Mode of action, applications, molecular biology, and epidemiology of bacterial resistance." Microbio. and Molec. Biol. Rev. 232-60. 

    Hubbard AR (2011)  Evaluation of Kasugamycin-use strategies designed to delay development of resistance in Erwinia amylovora. Mastes thesis. Botany and Plant Pathology, Oregon state University.

    MIC Bacillus cereus| ≥1|| Bacillus pumilus| 2|| Bacillus subtilis| ≥16|| Brochothrix thermosphacta| ≥124|| Burkholderia mallei| 16 - 32|| Clavibacter michiganensis subsp. michiganensis (185. 2. 1)| ≥4|| Diplococcus pneumoniae| 0.04 - 0.4|| Enterococcus faecalis| 0.78 - >100|| Enterococcus faecium| 0.39 - >100|| Enterococcus hirae| 0.39 - >100|| Enterococcus sp. (Uraguay)| ≤0.25 - 2|| Escherichia coli | 0.25 - 70|| Flavobacterium columnare| ≥15.63|| Fusobacterium necrophorum| 0.03 - 4.1|| Haemophilus influenzae| 1.6 - 6.3|| Haemophilus parasuis| 0.25 - 16|| Klebsiella pneumonia| ≥16|| Listeria monocytogenes| ≥58|| Micrococcus kristinae| 1 - 4|| Micrococcus luteus| ≥4|| Mycoplasma bovis | 0.12 - 128|| Mycoplasma gallisepticum| 0.05 - 200|| Mycoplasma hyopneumoniae| 0.03 - 4|| Mycoplasma hyosynoviae| 0.5 - >4|| Mycoplasma iowae| 0.025 - 100|| Mycoplasma meleagridis| 0.05 - 25|| Mycoplasma synoviae | 0.025 - 100|| Nocardia asteroides| 25 - 100|| Paenibacillus larvae| 0.016 - 0.031|| Pasteurella haemolytica| ≥64|| Proteus vulgaris| 16 - 64|| Pseudomonas aeruginosa | ≥8|| Pseudomonas flourescens| ≥40|| Salmonella enterica| ≥112|| Salmonella spp.| ≥2|| Shigella flexneri| ≥1|| Staphylococcus (coagulase-negative + Uruguay)| ≤0.25 - >64|| Staphylococcus aureus| ≤0.25 - 76|| Staphylococcus epidermidis| ≥4|| Streptococcus agalactiae| ≤0.25 - 2|| Streptococcus dysgalactiae | >4 - >64|| Streptococcus faecalis| ≥8|| Streptococcus iniae| ≥10|| Streptococcus pneumonia| 0.125 - 0.5|| Streptococcus uberis (Uruguay)| ≤0.25 - 64|| Treponema hyodysenteriae | 0.78 - 25|| Vibrio cholerae| ≥56|| Vibrio harveyi| ≥0.78||