SKU: C009  / 
    CAS Number: 123171-59-5

    Cefepime Hydrochloride

    $281.06

    Cefepime Hydrochloride is a broad-spectrum, fourth-generation cephalosporin antibiotic.  It is commonly used in antimicrobial susceptibility testing.  The addition of the hydrochloride salt enhances aqueous solubility.   Cefepime has potential for use as an antitumor agent, showing inhibition of human breast cancer cells in vitro when complexed with manganese.

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    Mechanism of ActionLike β-lactams, cephalosporins interfere with penicillin binding protein (PBP) activity involved in the final phase of peptidoglycan synthesis. PBP’s are enzymes which catalyze a pentaglycine crosslink between alanine and lysine residues providing additional strength to the cell wall. Without a pentaglycine crosslink, the integrity of the cell wall is severely compromised and ultimately leads to cell lysis and death. Resistance to cephalosporins is commonly due to cells containing plasmid encoded β-lactamases. Interestingly, Cefepime is resistant to various β-lactamases encoded by otherwise resistant β-lactam bacteria strains.
    SpectrumCefepime is a broad-spectrum antibiotic targeting a wide variety of naturally antibiotic resistant Gram-positive and Gram-negative bacteria. Some of these naturally resistant bacteria include Pseudomonas aeruginosa, Staphylococcus aureus, and Streptococcus pneumoniae.
    Microbiology ApplicationsCefepime Hydrochloride is commonly used in clinical in vitromicrobiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against Gram-positive and Gram-negative microbial isolates. Medical microbiologists use AST results to recommend antibiotic treatment options. Representative MIC values include:
    • Pseudomonas aeruginosa 32 µg/mL – 256 µg/mL
    • Staphylococcus aureus 2 µg/mL – 16 µg/mL
    • For a more complete list of cefepime MIC values, click here.

    Cefepime was used in a MALDI-TOF MS-based direct-on-target mirodroplet growth assay as part of a screening panel for rapid detection of ESBL, and AmpC β-Lactamases in Enterobacterales.  (Correa-Martinez et al, 2019). 

    Molecular FormulaC19H25ClN6O5S2 · HCl · H2O
    IdentificationE isomer: ≤0.2%
    Any individual impurity: ≤0.2%
    References

    Georgopapadakou NH and Bertasso A (1993)  Mechanisms of action of Cephalosporin 3'-quinolone esters, carbamates, and tertiary amines in Escherichia coli.  Antimicrob. Agents. Chemother. 37(3):559-565

    Zhang Z, Bi C, Fan Y, Wang H and Bao Y (2015) Cefepime, a fourth-generation cephalosporin, in complex with manganese, inhibits proteasome activity and induces the apoptosis of human breast cancer cells. Int. J. Molec. Med. 36(4): 1143-1150

    Cefepime from TOKU-E:

    Correa-Martinez C, Idelevich EA, Sparbier K, Kostrzewa M, and Becker K (2019)  Rapid detection of extended-spectrum B-lactamases (ESBL) and AmpC B-Lactamases in Enterobacterales: Development of a screening panel using the MALDI-TOF MS-based direct-on-target microdroplet growth assay. Front. Microbiol. 10:13  PMID 30733710

    MICBacillus cereus| 250 - >500|| Bacillus macerans (A199)| 15.62|| Bacillus pumilus| 31.25|| Bacillus subtilis| 62.5 - 125|| Brucella abortus (A77)| 62.5 - 125|| Cedecea davisae (F2)| 31.25|| Clavibacter michiganense (A227)| 15.62 - 16.62|| Enterobacter cloacae (A135)| 31.25|| Enterococcus faecalis (ATCC 29122)| 31.25 - 62.5|| Escherichia coli| 15.6 - 125|| Klebsiella pneumonia| 31.2 - 125|| Morganella morganii (F4)| 31.25|| Proteus mirabilis| 62.5|| Proteus vulgaris| 15.62 - 125|| Pseudomonas aeruginosa| 15.62 - >500|| Pseudomonas putida| 125|| Pseudomonas syringae| 31.25 - 125|| Salmonella cholerasuis arizonae (F7)| 62.5|| Salmonella enteritidis (ATCC 13076)| 31.25 - 62.5|| Salmonella typhi| >500|| Salmonella typhimurium| >500|| Serratia plymuthica (F8)| 31.25|| Shigella sonnei (F9)| 31.25|| Staphylococcus aureus| 7.8 - 62.5|| Staphylococcus epidermidis (A233)| 15.62 - 62.5|| Staphylococcus hominis (F10)| 125|| Streptococcus pyogenes| 31.25 - 125|| Xanthomonas campestris (A235)| 31.25|| Yersinia enterocolitica (F11)| 16.62||