Fluorescein Diacetate (syn: FDA) is an esterase substrate that can permeate cells and acts as a viability probe that measures both enzymatic activity (needed to activate its fluorescence) and cell-membrane integrity (required for intracellular retention of the fluorescent product). Upon hydrolysis by intracellular esterases, this compound yields fluorescein which is fluorescent green in living cells. Dead cells cannot accumulate or hydrolyse FDA and remain unstained. Fluorescein diacetate can be used as a fluorogenic substrate for hGSTP1-1.
FDA can be used in diagnostic assays, manufacturing, hematology, and histology.
FDA is soluble in water, ethanol, chloroform, dichloromethane or DMSO.
