SKU: A225-1G  / 
    CAS Number: 39831-55-5

    Amikacin Sulfate (1:1.8), AST grade

    $202.92
    Amikacin Sulfate (1:1.8), AST grade
    • Detailed Description

      CAS Number: 39831-55-5

      Molecular Formula: C22H43N5O13*1.8H2SO4

      Mechanism of Action: Amikacin Sulfate binds to the 30S ribosomal subunit (specifically the 16S rRNA and S12 protein) resulting in interference with the translational initiation complex and mRNA misreading, which leads to a faulty or nonexistent protein.

      Storage Conditions: 2-8°C

      Tariff Code: 2941.90.5000

    • Specifications

      Form: Powder

      Source: Semi-synthetic

    • References

      References:

      Chan K, Wang W , Ledesma KR, Yin T, and Tam VH (2020) A robust LC–MS/MS method for Amikacin: Application to cellular uptake and pharmacokinetic studies. Bioanalysis 12(7):445-454 PMID 32343148

      Christophe T et al (2009) High content screening identifies decaprenyl-phosphoribose 2’ epimerase as a target for intracellular antimycobacterial inhibitors. PLoS Pathog. 5(10): e10000645 PMID 19876393

      Davis, BD (1987) Mechanism of bactericidal action of aminoglycosides. Microbiol Rev 51 (3 ): 341-350 PMID 3312985

      Dudek M, Romanowska J, Witula T, Trylska J (2014) Interactions of Amikacin with the RNA model of the ribosomal A-site: Computational, spectroscopic and calorimetric studies. Biochimie 102:188-202 PMID 24769038

      Laurent G, Carlier MB, Rollman B, Van Hoof F, Tulkens P (1982) Mechanism of aminoglycoside-induced lysosomal phospholipidosis: In vitro and in vivo studies with gentamicin and Amikacin. Biochem. Pharmacol 31(23):3861-70 PMID 7159463

      Lo JY, Lim WW, Tam BK, Lai MY (1996) Vancomycin and Amikacin in cell cultures for virus isolation. Pathology 28(4):366-369 PMID 9007960

      Singh R, Ray P, Das A, Sharma MJ (2009) Role of persisters and small-colony variants in antibiotic resistance of planktonic and biofilm-associated Staphylococcus aureus: An in vitro study. Med. Microbiol 58(8):1067-1073. PMID 1952816

    Amikacin Sulfate (1:1.8), AST grade contains an Amikacin:Sulfate ratio of 1:1.8. Amikacin is a semi-synthetic, broad-spectrum aminoglycoside antibiotic derived from its counterpart, Kanamycin A. It was patented in 1971 and came into commercial use in 1976. The presence of the amino-hydroxy-butyryl group prevents enzymatic modification of Amikacin at multiple positions. This renders Amikacin a more effective decontaminating agent, as microorganisms which develop resistance to other aminoglycosides remain susceptible to Amikacin. This also gives Amikacin a broader spectrum than the other aminoglycosides currently in use. Amikacin is commonly used for veterinary research, antimicrobial susceptibility testing and studying bacterial translation.

    Amikacin Sulfate, USP (1:1.8) is soluble in water.

    This product has been optimized for antimicrobial susceptibilty testing (AST) applications.

    We also offer:

    • Amikacin Sulfate, USP (1:2) (A003)
    • Amikacin  (A002)

     

    CAS Number: 39831-55-5

    Molecular Formula: C22H43N5O13*1.8H2SO4

    Mechanism of Action: Amikacin Sulfate binds to the 30S ribosomal subunit (specifically the 16S rRNA and S12 protein) resulting in interference with the translational initiation complex and mRNA misreading, which leads to a faulty or nonexistent protein.

    Storage Conditions: 2-8°C

    Tariff Code: 2941.90.5000

    Form: Powder

    Source: Semi-synthetic

    References:

    Chan K, Wang W , Ledesma KR, Yin T, and Tam VH (2020) A robust LC–MS/MS method for Amikacin: Application to cellular uptake and pharmacokinetic studies. Bioanalysis 12(7):445-454 PMID 32343148

    Christophe T et al (2009) High content screening identifies decaprenyl-phosphoribose 2’ epimerase as a target for intracellular antimycobacterial inhibitors. PLoS Pathog. 5(10): e10000645 PMID 19876393

    Davis, BD (1987) Mechanism of bactericidal action of aminoglycosides. Microbiol Rev 51 (3 ): 341-350 PMID 3312985

    Dudek M, Romanowska J, Witula T, Trylska J (2014) Interactions of Amikacin with the RNA model of the ribosomal A-site: Computational, spectroscopic and calorimetric studies. Biochimie 102:188-202 PMID 24769038

    Laurent G, Carlier MB, Rollman B, Van Hoof F, Tulkens P (1982) Mechanism of aminoglycoside-induced lysosomal phospholipidosis: In vitro and in vivo studies with gentamicin and Amikacin. Biochem. Pharmacol 31(23):3861-70 PMID 7159463

    Lo JY, Lim WW, Tam BK, Lai MY (1996) Vancomycin and Amikacin in cell cultures for virus isolation. Pathology 28(4):366-369 PMID 9007960

    Singh R, Ray P, Das A, Sharma MJ (2009) Role of persisters and small-colony variants in antibiotic resistance of planktonic and biofilm-associated Staphylococcus aureus: An in vitro study. Med. Microbiol 58(8):1067-1073. PMID 1952816