• Ciprofloxacin hydrochloride packaged and labeled.

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SKU: C032

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Description

Ciprofloxacin hydrochloride is a broad-spectrum second generation 4-fluoroquinolone antibiotic that targets the bacterial enzyme DNA gyrase and is effective against Gram-positive and Gram-negative bacteria and Mycoplasmas.  Ciprofloxacin HCl is freely soluble in aqueous solution (35 mg/mL).

TOKU-E offers two forms of Ciprofloxacin:

    CAS Number

    86393-32-0

    Molecular Formula

    C17H18FN3O3 · HCl· H2O

    Molecular Weight

    385.82 g/mol

    Mechanism of Action

    Fluoroquinolone antibiotics target bacterial DNA gyrase (topoisomerase type II), an enzyme which reduces DNA strain during replication and is essential for DNA packaging, transcription, and replication. Thus, DNA synthesis and cell division is inhibited.

    Storage Conditions

    2-8 °C, protected from light.

    Tariff Code

    2933.59.3600

    Spectrum

    Ciprofloxacin is a broad spectrum antibiotic targeting a wide variety of Gram-positive and Gram-negative bacteria. It is also effective against Mycoplasmas.

Applications

    Cancer Applications

    Two transitional cell carcinoma cell lines, MBT‐2 and T24, were used in an in vitro study of the effects of ciprofloxacin on cell proliferation, and the compound was found to inhibit cell proliferation in a dose-dependent manner (Ebisuno et al, 1997).

    Eukaryotic Cell Culture Applications

    Mycoplasma-infected leukemia cell lines were cultured with 10 ug/ml ciprofloxacin for 14 days (Gignac et al 1991). Murine plasmocytoma cell lines X63-Ag8 653 were experimentally infected with seven different species of Mycoplasma and eradication was found with 10 ug/ml ciprofloxacin. Wild-type lab infections of human cell lines HL-60 and U-937 were eliminated with 12 days of treatment. No side effects were seen in cell cultures (Schmitt et al, 1988).

    Microbiology Applications

    Ciprofloxacin HCl is commonly used in clinical in vitro microbiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against gram positive and gram negative microbial isolates. Medical microbiologists use AST results to recommend antibiotic treatment options for infected patients.

    • For a complete list of ciprofloxacin MIC values, click here.

    Plant Biology Applications

    Garlic protoplasts were cultured in medium containing ciprofloxacin, and the compound was successful at preventing microbial growth and was not toxic to the protoplasts. (Fellner, 1995)

Specifications

    Form

    Powder

    Appearance

    White or off-white crystalline powder

    Source

    Synthetic

    Water Content (Karl Fischer)

    4.7-6.7%

    pH

    3.0-4.5

    Assay

    98.0-102.0% (on dry basis)

    Residue On Ignition

    ≤0.1%

    Heavy Metals

    ≤0.002%

    Impurities

    Sulfate: ≤ 0.04%
    Fluoroquinolinic acid: ≤0.2%
    Ciprofloxacin ethylenediamine analog: ≤0.2%
    Any other impurity: ≤0.2% Total Impurities: ≤0.5%

References

    References

    Ebisuno S, Inagaki T, Kohjimoto Y and Ohkawa T (1997) The cytotoxic effects of fleroxacin and ciprofloxacin on transitional cell carcinoma in vitro. Cancer 80(12):2263-2267 PMID 9404703

    Fellner M (1995) Influence of the antibiotic ciprofloxacin on culture of Allium longicuspis callus-derived protoplasts. Ann. Bot. 76: 219-223

    Gignac SM, Brauer S, Hane B, Quentmeier H, and Drexler HG (1991) Elimination of mycoplasma from infected leukemia cell lines. Leukemia 5(2):162-165 PMID 2020199

    Ridgway GL, Mumtaz G, Gabriel FG, and Oriel JD (1986) The Activity of Ciprofloxacin and Other 4-Quinolones Against Chlamydia trachomatis and Mycoplasmas In Vitro. In: Neu HC, Reeves DS. (eds) Ciprofloxacin. Current Topics in Infectious Diseases and Clinical Microbiology, vol 1. Vieweg+Teubner Verlag, Wiesbaden

    Schmitt K, Däubener W, Bitter-Suermann D, Hadding U.(1988) A safe and efficient method for elimination of cell culture mycoplasmas using ciprofloxacin. 109(1):17-25 PMID 3282011

    Wolfson, JS and Hooper DC (1985) The Fluoroquinolones: Structures, Mechanisms of Action and Resistance, and Spectra of Activity in Vitro. Antimicrob Agents Chemother. 28(4):581-586 PMID 3000292

    Zeiler HJ and Grohe K. (1986) The In Vitro and In Vivo Activity of Ciprofloxacin. In: Neu HC, Reeves DS (eds) Ciprofloxacin. Current Topics in Infectious Diseases and Clinical Microbiology, vol 1. Vieweg+Teubner Verlag, Wiesbaden

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