• Kanamycin A sulfate, EvoPure packaged and labeled.

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SKU: K013

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Description

Kanamycin is an aminoglycoside antibiotic often used to select for plants (Agrobacterium mediated transformation) and bacteria which have been successfully transformed with a plasmid conferring kanamycin resistance.  Kanamycin is very soluble in aqueous solution at 92.3 mg/mL.

Standard grade kanamycin is composed of a mixture of three different fractions: kanamycin A, B, and C. TOKU-E offers five forms of kanamycin:

EvoPure® products are purified single antibiotic fractions, most >99% pure. High purity EvoPure® kanamycin products can be used to analyze the specific effects of individual kanamycin fractions.

    CAS Number

    25389-94-0

    Molecular Formula

    C18H36N4O11· xH2SO4 (lot specific)

    Molecular Weight

    484.50 g/mol (Free base)

    Mechanism of Action

    Aminoglycosides target the 30S ribosomal subunit resulting in an inability to read mRNA ultimately producing a faulty or nonexistent protein.

    Storage Conditions

    2-8 °C

    Tariff Code

    2941.90.1010

    Spectrum

    Kanamycin is a broad spectrum antibiotic, however, it is mostly used against aerobic Gram-negative bacteria.

Applications

    Microbiology Applications

    Kanamycin is commonly used in clinical in vitro microbiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against gram positive and gram negative microbial isolates. Medical microbiologists use AST results to recommend antibiotic treatment options for infected patients. Representative MIC values include:

    • Salmonella enteritidis 62.5 µg/mL
    • Escherichia coli 31.2 µg/mL
    • For a complete list of kanamycin MIC values, click here.

    Media Supplements

    Kanamycin can be used as a selective agent in several types of isolation media:

    Kanamycin Aesculin Azide AgarEnterococci isolation in food

    Plant Biology Applications

    Kanamycin is often used in Agrobacterium mediated transformation using the npt II gene as selection marker. Kaur and Bansal (2010) used kanamycin in combination with cefotaxime to control bacterial growth while transforming tomato.

Specifications

    Form

    Powder

    Appearance

    White powder

    Source

    Streptomyces Kanamyceticus

Technical Data

HPLC Chromatogram Showing Ultra High, Single Fraction Purity of Kanamycin A Sulfate, EvoPure®

Kanamycin A Sulfate EvoPure

References

    References

    Davis, Bernard D. "Mechanism of Bactericidal Action of Aminoglycosides."Microbiological Reviews 51.3 (1987): 341-50.

    Aragão F.J.L. and Brasileiro A.C.M., 2009 Positive, negative and marker-free strategies for transgenic plant selection. Braz. J. Plant Physiol., 14(1):1-10, 2002

    Sharma M.K., Solanke A. U., Jani D., Singh Yogendra and Sharma A.K. A simple and efficient Agrobacterium-mediated procedure for transformation of tomato. J. Biosci. 34(3), September 2009, 423–433.Aragão F.J.L. and Brasileiro

    Kaur P., and Bansal K.C. Efficient production of transgenic tomatoes via Agrobacterium-mediated transformation. Biologica plantarum 54 (2): 344-348, 2010

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