• Kanamycin B sulfate, EvoPure packaged and labeled.

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SKU: K014

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Kanamycin is an aminoglycoside antibiotic often used to select for bacteria which have been successfully transformed with a plasmid conferring kanamycin resistance.

Kanamycin B sulfate, EvoPure® is ≥99.0% pure by HPLC.

Standard grade kanamycin is composed of a mixture of three different fractions: Kanamycin A, B, and C.  TOKU-E offers five forms of kanamycin:

EvoPure® products are purified single antibiotic fractions, most >99% pure. High purity EvoPure® kanamycin products can be used to analyze the specific effects of individual kanamycin fractions.

Huang et al. used kanamycin B sulfate, EvoPure® to study the roles of oxidoreductase GenD2 and transaminase GenS2. "Delineating the Biosynthesis of Gentamicin X2, the Common Precursor of the Gentamicin C Antibiotic Complex."

    CAS Number


    Molecular Formula

    C18H37N5O10 · xH2SO4 (lot specific)

    Molecular Weight

    483.51 g/mol (Free base)

    Mechanism of Action

    Aminoglycosides target the 30S ribosomal subunit resulting in an inability to read mRNA ultimately producing a faulty or nonexistent protein.

    Storage Conditions


    Tariff Code



    Kanamycin is a broad spectrum antibiotic, however, it is mostly used against aerobic gram negative bacteria.


    Microbiology Applications

    Kanamycin is commonly used in clinical in vitro microbiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against gram positive and gram negative microbial isolates. Medical microbiologists use AST results to recommend antibiotic treatment options for infected patients. Representative MIC values include:

    • Salmonella enteritidis 62.5 µg/mL
    • Escherichia coli 31.2 µg/mL
    • For a complete list of kanamycin MIC values, click here.

    Plant Biology Applications

    Kanamycin is often used in the Agrobacterium mediated transformation while using the npt II gene as selection marker. Kaur and Bansal (2010) used kanamycin in combination with cefotaxime to control bacterial growth while transforming tomato.





    White to off-white crystalline powder



Technical Data

HPLC, NMR, FTIR, and MS analysis may be available. For more info, please email [email protected].



    Davis, Bernard D. "Mechanism of Bactericidal Action of Aminoglycosides."Microbiological Reviews 51.3 (1987): 341-50.

    United States. National Institutes of Health. Kanamycin Compound SummaryPubChem. Web. 21 Aug. 2012.

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