• Kanamycin B sulfate, EvoPure packaged and labeled.

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SKU: K014

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Description

Kanamycin B Sulfate, EvoPure® is the highly purified sulfate salt form of Kanamycin B.  The product is ≥99.0% pure by HPLC.  Kanamycin B is a minor component of the Kanamycin complex, along with Kanamycin C, while Kanamycin A is the major component. 

Kanamycn B is a close structural analog of DAA2 (what is this?) and it has a n unmethylated 3’-amino group so it can be tested as a surrogate substrate for 3’-deamination.

Kanamycin is a semi-synthetic, broad-spectrum aminoglycoside antibiotic.  Kanamycin was isolated from the soil bacterium Streptomyces kanamyceticus in 1957 by Hamao Umezawa at the institute of Microbial Chemistry (Tokyo).  Kanamycin inhibits protein synthesis in bacteria and Mycoplasma.  It is used to select for transformants (bacteria, fungi, plant, mammalian) which have been successfully transformed with a plasmid containing a kanamycin resistance gene. 

Kanamycin B Sulfate, EvoPure® is freely soluble in water. It is practically insoluble in ethanol, DMSO, acetone, chloroform, ether and ethyl acetate.

We also offer:

  • Kanamycin Sulfate (K008)
  • Kanamycin Acid Sulfate, BP (K004)
  • Kanamycin Acid Sulfate, EP (K029)
  • Kanamycin A sulfate, EvoPure® (sKU)

Kanamycin EvoPure® products are available as highly purified fractions (see Technical Data).  Each compound is fully characterized by spectral analysis and a comprehensive C of A is provided which includes HPLC, MS, HNMR and FTIR data.

    CAS Number

    29701-07-3

    Molecular Formula

    C18H37N5O10 · xH2SO4 (lot specific)

    Molecular Weight

    483.51 g/mol (Free base)

    Mechanism of Action

    Aminoglycosides target the 30S ribosomal subunit, lodging between the 16S rRNA and S12 protein. This interferes with the translational initiation complex causing misreading of the mRNA, and production of a faulty or nonexistent protein.

    Storage Conditions

    -20°C

    Tariff Code

    2941.90.1010

    Spectrum

    Kanamycin is a broad-spectrum antibiotic, however, it is mostly used against aerobic Gram-negative bacteria.

Applications

    Microbiology Applications

    Kanamycin is commonly used in clinical in vitro microbiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against Gram-positive and Gram-negative microbial isolates. Medical microbiologists use AST results to recommend antibiotic treatment options.  Representative MIC values include:

    • E. coli 3.125 µg/mL
    • P. aeruginosa  3.125 µg/mL
    • For a representative ist of Kanamycin B MIC values, click here.

    Kanamycin B was used as a structural analog of DAA2, an important enzyme in the Gentamicin biosynthetic pathway, to study the use of the biosynthetic pathway of Gentamicin to gain insights into the engineering safer Aminoglycoside derivatives (Huang et al, 2015).

    Kanamycin is one of the most widely used antibiotics yet its biosynthetic pathway remains unclear. Authors reconstructed the entire biosynthetic pathway through the expression of putative biosynthetic genes from S. kanamyceticus in the non-aminoglycoside-producing Streptomyces venezuelae, demonstrating the potential of pathway engineering to direct in vivo production of more robust aminoglycosides (Park et al, 2011).

    Plant Biology Applications

    Kanamycin is often used in Agrobacterium mediated transformation using the npt II gene as selection marker.

Specifications

    Form

    Powder

    Appearance

    White to off-white crystalline powder

    Source

    Semi-biosynthetic

Technical Data

HPLC, NMR, FTIR, and MS analysis may be available. For more info, please email [email protected].

References

    References

    Product Reference

     Huang et al. used TOKU-E Kanamycin B Sulfate, EvoPure® to study the roles of oxidoreductase GenD2 and transaminase GenS2. "Delineating the Biosynthesis of Gentamicin X2, the Common Precursor of the Gentamicin C Antibiotic Complex."

    G
    eneral References

    Aragão FJL and Brasileiro ACM (2009) Positive, negative and marker-free strategies for transgenic plant selection. Braz. J. Plant Physiol. 14(1):1-10

    Davis BD (1987)  Mechanism of bactericidal action of Aminoglycosides.  Microbiol. Rev.  51(3):341-350

    Huang C, Huang F and Sun Y (2015)  Delineating the biosynthesis of Gentamicin X2, the common precursor of the Gentamicin C antibiotic complex.  Chem Biol 22(2):251-261  PMID 25641167

    Park JW et al (2011)  Discovery of parallel pathways of kanamycin biosynthesis allows antibiotic manipulation. Nat. Chem. Biol. 7(11):843-852 PMID 21983602

    Sneader W (2005)  Drug Discovery: A history. John Wiley & Sons pp 302


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