• Kanamycin acid sulfate packaged and labeled.

click on thumbnail to zoom
SKU: K004

Size  
Quantity:
  Click 'Bulk Order' for 10 or more
Price: make selection(s)
Description

Kanamycin Acid Sulphate, BP is a broad-spectrum aminoglycoside antibiotic often used to select for bacteria which have been successfully transformed with a plasmid conferring kanamycin resistance.  

It is derived through solubilization of Kanamycin Sulfate in dilute sulfuric acid and then drying.  The sulfate content of the product is approximately 25%.   

Kanamycin Acid Sulphate, BP is soluble in water.  It is practically insoluble in ethanol, acetone, chloroform, ether, and ethyl acetate. 

Kanamycin Acid Sulfate, BP conforms to British Pharmacopoeia specifications. 

We also offer:

  • Kanamycin Sulfate, USP (K008)
  • Kanamycin A sulfate, EvoPure® (K013)
  • Kanamycin B sulfate, EvoPure® (K014)

    CAS Number

    64013-70-3

    Molecular Formula

    C18H36N4O11 · 2 H2SO4

    Molecular Weight

    680.65 g/mol

    Mechanism of Action

    Aminoglycosides target the 30S ribosomal subunit, lodging between the 16S rRNA and S12 protein. This interferes with the translational initiation complex causing misreading of the mRNA, and production of a faulty or nonexistent protein. Resistance to aminoglycosides is due to aminoglycoside-modifying enzymes (including acetyltransferase, phosphotransferase, nucleotidyltransferase) which can alter this antibiotic.

    Storage Conditions

    2-8 °C

    Tariff Code

    2941.90.1010

    Spectrum

    Kanamycin is a broad-spectrum antibiotic; however, it is mostly used against aerobic Gram-negative bacteria. It can be used against Mycoplasma and Mycobacteria.

Applications

    Microbiology Applications

    Kanamycin Acid Sulfate is commonly used as a selective agent to select for resistant mammalian, fungal, or bacterial cells that contain the kanMX marker or other kanamycin resistance genes. Kanamycin Acid Sulfate is typically used at a concentration of 50 µg/mL

    Media Supplements

    Kanamycin can be used as a selective agent in several types of isolation media:

    Kanamycin Aesculin Azide Agar - Enterococci isolation in food

    Perfringens Agar - SFP and TSC selective supplements for the isolation of Clostridium perfringens

    Kanamycin is commonly used in clinical in vitro microbiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against Gram-positive and Gram-negative microbial isolates. Medical microbiologists use AST results to recommend antibiotic treatment options. 

    Plant Biology Applications

    Kanamycin is often used in the Agrobacterium mediated transformation while using the npt II gene as selection marker. Microbial cellulosic utilization makes use of Kanamycin in gene selection for purpose of bioprocessing of cellulosic biomass for conversion into biofuels. Strategies include engineering naturally occurring microorganisms to improve yield/titer, or engineering non-cellulolytic organisms to express a heterologous cellulase system (Lynd et al, 2005).

    Caldicellulosiruptor bascii is a thermophilic anaerobic bacterium that can deconstruct non-pretreated lignocellulosic plant biomass and is the most thermophilic cellulose degrader known.  It is of interest in genetic engineering applications for biofuel production.  A thermostable Kanamycin resistance gene (Cbhtk) was used in the study.  A selection system was developed using kanamycin that can be used to efficiently delete genes, with the goal of producing a thermostable version of lactate dehydrogenase (Lipscomb et al, 2016).

Specifications

    Form

    Powder

    Appearance

    White or almost white powder

    Source

    Streptomyces kanamyceticus

    Potency (on a dry basis)

    ≥670 IU/mg

    pH

    5.5-7.5

    Loss on Drying

    ≥5.0%

    Sulfated Ash

    ≥5.0%

    Impurities

    Kanamycin B: ≤3.6%

References

    References

    Product Reference:

    Pryjma, et al. used Kanamycin Sulfate from TOKU-E to select for transformed Kanamycin-resistant Campylobacter jejuni cells: "FdhTU-modulated formate dehydrogenase expression and electron donor availability enhance recovery of Campylobacter jejuni following host cell infection"


    General References:

    Aragão FJL and Brasileiro ACM (2009) Positive, negative and marker-free strategies for transgenic plant selection. Braz. J. Plant Physiol. 14(1):1-
    10

    Bastian I, Rigouts L, Palomino JC and Portaels F (2001)  Kanamycin Susceptibility Testing of Mycobacterium tuberculosis Using Mycobacterium Growth Indicator Tube and a Colorimetric Method. Antimicrob. Agents Chemother. 45(6):1934-1936

    Davis, BD (1987)  Mechanism of bactericidal action of aminoglycosides.  Microbiol. Rev. 51(3):341-350

    Lipscomb GL et al (2016)  A highly thermostable Kanamycin resistance marker expands the tool kit for genetic manipulation of Caldicellulosiruptor bescii.  Appl. Environ. Microbiol. 82 (14): 4421-4428

    Lynd LR, van Zyl WH, McBride JE and Laser M (2005)  Consolidated bioprocessing of cellulosic biomass: An update. Curr. Opin. Biotechnol. 16(5):577-583

    Park JW et al (2011)  Discovery of parallel pathways of kanamycin biosynthesis allows antibiotic manipulation. Nat. Chem. Biol. 7(11):843-852 PMID 21983602

    Zanzotto A et al (2004)  Membrane-aerated microbioreactor for high-throughput bioprocessing.  Biotechnol. Bioeng. 87(2):243-254

Other Items In This Category