• Gentamicin C2a sulfate, EvoPure packaged and labeled.

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SKU: G034

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Description

Gentamicin C2a sulfate, EvoPure® is ≥95.0% pure Gentamicin C2a and can be used to study the Gentamicin biosynthetic pathway. Gentamicin C2a is a component of the Gentamicin C complex, along with Gentamicin C1 and Gentamicin C1a, and together this complex makes up 80% of the compound and has the highest antibacterial activity.  Gentamicin C2a and C2 are stereoisomers.  The higher antimicrobial activity of the complex is thought to arise from the lack of hydroxy groups on the 3’ and 4’ positions of the purpurosamine (2-amino-hexose) fragments.

For more Gentamicin products, click here.

    CAS Number

    38539-12-7; 59751-72-3 (free base)

    Molecular Formula

    C20H41N5O· xH2SO4 (lot specific)

    Molecular Weight

    463.57 g/mol (Free base)

    Mechanism of Action

    Aminoglycosides target the 30S ribosomal subunit resulting in an inability to read mRNA ultimately producing a faulty or nonexistent protein.

    Storage Conditions

    -20°C

    Tariff Code

    2941.90.1010

    Spectrum

    Gentamicin is a broad-spectrum antibiotic targeting a wide variety of Gram-positive and Gram-negative bacteria. It is effective against several strains of Mycoplasma.

Applications

    Microbiology Applications

    Gentamicin EvoPure® compounds can be used to study effects of individual Gentamicin components on various bacterial strains.

      Representative MIC values include:

    • Bacillis subtilis ATCC 6633: 0.02 µg/mL
    • Escherichia coli ATCC 10536: 2.2µg/mL
    • Staphylococcus aureus ATCC 6538P 1.0 µg/mL

Specifications

    Form

    Powder

    Appearance

    White or off-white powder

    Source

    Micromonospora spp.

    pH

    3.5-5.5

Technical Data

Spectral Data - Gentamicin C2a sulfate, EvoPure®


HNMR Spectra


      

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Solvent: D2O
Instrument: Mercury 300
Frequency: 300 MHz

 

FTIR Spectra

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Mass Spectra

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Polarity/Scan Type: Positive
Solvent: H2O
Solution Concentration: 10 mg/mL
References

    References

    Davis, BD (1987) Mechanism of bactericidal action of aminoglycosides. Microbiol. Rev. 51(3): 341-350 PMID 3312985

    Stypulkowska K, Blazewicz A, Fijalek Z, Sarna K.(2010) Determination of gentamicin sulphate composition and related substances in pharmaceutical preparations by LC with charged aerosol detection. Chromatograph. 72(11-12):1225-1229 PMID 21212825

    Vydrin, AF (2003) Component composition of gentamicin sulfate preparations. Pharma. Chem. J 37(8): 448-449

    Weinstein, MJ, Wagman GH, Oden EM and Marquez JA (1967) Biological activity of the antibiotic components of the gentamicin complex. J. Bacteriol. 94(3):789-790 PMID 4962848

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