• Gentamicin C1 sulfate, EvoPure packaged and labeled.

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SKU: G031

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Description

Gentamicin C1 sulfate is one of several components of the gentamicin C complex. Gentamicin C1 can comprise 25-50% gentamicin depending on the manufacturer or manufacturing process. The gentamicin C complex comprises approximately 80% gentamicin and exhibits the most potent antimicrobial activity. The higher antimicrobial activity of the group C gentamicins is thought to arise from the lack of hydroxy groups on the 3’ and 4’ positions of the purpurosamine (2-amino-hexose) fragments. Gentamicin C1 differs from gentamicin C1a by a methyl group in the 6’ position of the purpurosamine (2-amino-hexose) ring. Gentamicin C1 sulfate, EvoPure® is ≥95.0% gentamicin C1.

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Caldwell et al. used gentamicin C1 sulfate from TOKU-E to study aminoglycoside binding sites of APH-GMPPNP and APH-GTP crystals. "Antibiotic Binding Drives Catalytic Activation of Aminoglycoside Kinase APH(2″)-Ia."  

    CAS Number

    25876-10-2

    Molecular Formula

    C21H43N5O· xH2SO4 (lot specific)

    Molecular Weight

    477.59 g/mol (Free base)

    Mechanism of Action

    Aminoglycosides target the 30S ribosomal subunit resulting in an inability to read mRNA ultimately producing a faulty or nonexistent protein.

    Storage Conditions

    -20°C

    Tariff Code

    2941.90.1010

    Spectrum

    Gentamicin is a broad spectrum antibiotic and is active against several Gram-positive and Gram-negative bacteria.

Applications

    Microbiology Applications

    Gentamicin EvoPure® compounds can be used to study effects of individual gentamicin components on various bacterial strains.

      Representative MIC values include:

    • Bacillis subtilis ATCC 6633: 0.02 µg/mL
    • Escherichia coli ATCC 10536: 0.9µg/mL
    • Staphylococcus aureus ATCC 6538P 0.05 µg/mL

Specifications

    Form

    Powder

    Appearance

    White powder

    Source

    Micromonospora spp.

    pH

    3.5-5.5

Technical Data

Spectral Data - Gentamicin C1 sulfate, EvoPure®


HNMR Spectra


      

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Solvent: D2O
Instrument: Mercury 300
Frequency: 300 MHz

 

FTIR Spectra

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Mass Spectra

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Polarity/Scan Type: Positive
Solvent: Water
Solution Concentration: 10 mg/mL
Instrument: Agilent
References

    References

    Davis, Bernard D. "Mechanism of Bactericidal Action of Aminoglycosides."Microbiological Reviews 51.3 (1987): 341-50.

    Sarna, Katarzyna, and Agata Blazewicz. "Determination of Gentamicin Sulphate Composition and Related Substances in Pharmaceutical Preparations by LC with Charged Aerosol Detection." PubMed (2010)

    Vydrin, A. F. "Component Composition of Gentamicin Sulfate Preparations." Pharmaceutical Chemistry Journal 37.8 (2003): 448-49. Web. 15 Oct. 2013.

    Weinstein, Marvin J. "Biological Activity of the Antibiotic Components of the Gentamicin Complex." Journal of Bacteriology 94.3 (1967): 789-90. Web. 15 Oct. 2013.

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