• Gentamicin C2 sulfate, EvoPure packaged and labeled.

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SKU: G033

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Gentamicin C2 sulfate is one of several components of the gentamicin C complex. Gentamicin C2 can comprise 25-55% gentamicin depending on the manufacturer or manufacturing process. The gentamicin C complex comprises approximately 80% gentamicin and exhibits the most potent antimicrobial activity. The higher antimicrobial activity of the group C gentamicins is thought to arise from the lack of hydroxy groups on the 3’ and 4’ positions of the purpurosamine (2-amino-hexose) fragments. Gentamicin C2 and C2a are stereoisomers. Gentamicin C2 sulfate, EvoPure® is ≥95.0% gentamicin C2.

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    CAS Number

    25876-11-3 (free base)

    Molecular Formula

    C20H41N5O· xH2SO(lot specific)

    Molecular Weight

    463.57 g/mol (Free base)

    Mechanism of Action

    Aminoglycosides target the 30S ribosomal subunit resulting in an inability to read mRNA ultimately producing a faulty or nonexistent protein.

    Storage Conditions


    Tariff Code



    Gentamicin is a broad spectrum antibiotic targeting a wide variety of gram positive and gram negative bacteria.


    Microbiology Applications

    Gentamicin EvoPure® compounds can be used to study effects of individual gentamicin components on various bacterial strains.

      Representative MIC values include:

    • Bacillis subtilis ATCC 6633: 0.02 µg/mL
    • Escherichia coli ATCC 10536: 1.0µg/mL
    • Staphylococcus aureus ATCC 6538P 0.06 µg/mL





    White or off-white powder


    Micromonospora spp.


    3.5-5.5 (4% in H2O)

Technical Data

Spectral Data - Gentamicin C2 sulfate, EvoPure®

HNMR Spectra


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Solvent: D2O
Instrument: Varian 300
Frequency: 300 MHz


FTIR Spectra

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    Davis, Bernard D. "Mechanism of Bactericidal Action of Aminoglycosides."Microbiological Reviews 51.3 (1987): 341-50.

    Sarna, Katarzyna, and Agata Blazewicz. "Determination of Gentamicin Sulphate Composition and Related Substances in Pharmaceutical Preparations by LC with Charged Aerosol Detection." PubMed (2010)

    Vydrin, A. F. "Component Composition of Gentamicin Sulfate Preparations." Pharmaceutical Chemistry Journal 37.8 (2003): 448-49. Web. 15 Oct. 2013.

    Weinstein, Marvin J. "Biological Activity of the Antibiotic Components of the Gentamicin Complex." Journal of Bacteriology 94.3 (1967): 789-90. Web. 15 Oct. 2013.

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