• Clindamycin hydrochloride packaged and labeled.

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SKU: C035

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Description

Clindamycin Hydrochloride is a broad-spectrum antibiotic and antiparasitic agent.  It is a semi-synthetic derivative of Lincomycin, a natural lincosamide isolated from Streptomyces lincolnensis in 1966.  Clindamycin Hydrochloride is freely soluble in water.

We also offer:

  • Clindamycin (C233)
  • Clindamycin Phosphate (C036)

    CAS Number

    21462-39-5

    Molecular Formula

    C18H33ClN2O5S · HCl

    Molecular Weight

    461.44 g/mol

    Mechanism of Action

    Lincosamides inhibit bacterial protein synthesis by binding the 50S ribosomal subunit and interfering with tRNA activity during translation.

    Storage Conditions

    2-8 ┬░C. Store in airtight container.

    Tariff Code

    2941.90.5000

    Spectrum

    Clindamycin is a broad-spectrum antibiotic targeting primarily Gram-positive and Gram-negative bacteria such as Clostridium and Bacteroides species.

Applications

    Eukaryotic Cell Culture Applications

    HIV-infected cells (MOLT3 cells) were incubate with clindamycin in increasing concentrations, and did not result in increased cell death compared to uninfected control cells. (Wijsman et al, 2013).

    The effects of clindamycin and its metabolites on mammalian cell lines (mouse leukemia L1210, human oral carcinoma KB, human acute myelogenous leukemia RPMI 6410, and human lymphocyte RPMI 1788) were evaluated.   Metabolites clindamycin sulfoxide and clindamycose were nontoxic, wherease N-demethyl clindamycin showed cytotoxic effects in culture (Li et al, 1977).

    Microbiology Applications

    Clindamycin is commonly used in clinical in vitromicrobiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against gram positive and gram negative anaerobes.  Medical microbiologists use AST results to recommend antibiotic treatment options. Representative ranges include:

    • Clostridium difficile 0.25 µg/mL - 32 µg/mL
    • Bacteroides fragilis 0.25 µg/mL - 4 µg/mL

    For a complete list of Clindamycin MIC values, click here.

Specifications

    Form

    Powder

    Appearance

    White or almost white crystalline powder

    Source

    Semi-synthetic

    Water Content (Karl Fischer)

    3.0-6.0%

    Potency (on a dry basis)

    ≥800 µg/mg (C18H33ClN2O5S)

    pH

    3.0-5.5

References

    References

    Dhawan VK and Thadepalli H. (1982)  Clindamycin: A review of fifteen years of experience. Clin. Infect. Dis. 4(6):1133-1153  PMID 6818656

    Li LH, Kuentzel K L, Shugars KD and Bhuyan BK (1977)  Cytotoxicity of several marketed antibiotics on mammalian cells in culture. J. Antibiot (Tokyo) 30(6):506-512  PMID 560364

    Lovmar, M and Tanel T (2003)  The Mechanism of action of macrolides, lincosamides and streptogramin B reveals the nascent peptide exit path in the ribosome. J. Molec. Microbiol.  330(5 ): 1005-014  PMID 12860123

    Magerlein BJ et al (1966)  Chemical modification of lincomycin. Antimicrob Agents Chemother. 6:727 PMID 5985307

    Wijsman JA, Dekaban GA and Rieder MJ (2013)  Differential toxicity of reactive metabolites of clindamycin and sulfonamides in HIV-infected cells: Influence of HIV infection on clindamycin toxicity in vitro. J. Clin. Pharmacol. 45(3):346-351  PMID 15703369

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