• Cefuroxime sodium packaged and labeled.

click on thumbnail to zoom
SKU: C023

Size  
Quantity:
  Click 'Bulk Order' for 10 or more
Price: make selection(s)
Description

Cefuroxime Sodium is a broad-spectrum, semi-synthetic second-generation cephalosporin antibiotic with increased stability to beta-lactamases.  It is bactericidal against Gram-negative and Gram-positive bacteria.  Cefuroxime sodium is freely soluble in aqueous solution (0.145 mg/mL).

    CAS Number

    56238-63-2

    Molecular Formula

    C16H15N4NaO8S

    Molecular Weight

    446.37 g/mol

    Mechanism of Action

    Like β-lactams, cephalosporins interfere with PBP (penicillin binding protein) activity involved in the final phase of peptidoglycan synthesis. PBP’s are enzymes which catalyze a pentaglycine crosslink between alanine and lysine residues providing additional strength to the cell wall. Without a pentaglycine crosslink, the integrity of the cell wall is severely compromised and ultimately leads to cell lysis and death. Resistance to cephalosporins is commonly due to cells containing plasmid encoded β-lactamases. Like many second generation cephalosporins, Cefuroxime is relatively stable in the presence of β-lactamases.

    Storage Conditions

    -20°C

    Tariff Code

    2941.90.5000

    Spectrum

    Cefuroxime sodium is a broad-spectrum antibiotic targeting a wide variety of Gram-negative and Gram-positive bacteria.

Applications

    Eukaryotic Cell Culture Applications

    Rat erythrocyte glucose-6-phosphate dehydrogenase was found to increase in activity in response to Cefuroxime exposure in vitro (Temel Y et al, 2018). 

    Cell proliferation, cytotoxicity, cell metabolism, and extracellular matrix calcification were assessed after antibiotic treatment. Cefuroxime had no inhibiting effects on osteoblasts with lower concentrations (25-100 ug/ml), but higher concentrations (250-1000 ug/ml) saw an increase in cell proliferation and cytotoxicity, and an inhibition of cell metabolism (Salzmann GM et al, 2007).

    Microbiology Applications

    Cefuroxime sodium is commonly used in clinical in vitro microbiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against Gram-positive and Gram-negative microbial isolates. Medical microbiologists use AST results to recommend antibiotic treatment options for infected patients. Representative MIC values include:

    • Haemophilus influenzae 0.25 µg/mL – 4 µg/mL
    • Neisseria gonorrhoeae 0.2 µg/mL – 1.6 µg/mL
    • For a complete list of Cefuroxime MIC values, click here.

Specifications

    Form

    Powder

    Appearance

    White to yellowish crystalline powder

    Source

    Synthetic

    Impurity Profile

    Individual Impurity: Not more than 1.0%
    Total Impurities: Not more than 3.0%

    Water Content (Karl Fischer)

    ≤3.5%

    pH

    6.0-8.5

    Optical Rotation

    +55° to +65º

    Assay

    (Dried Basis): Not less than 86.0%

References

    References

    Georgopapadakou, NH (1992) Mechanisms of action of cephalosporin 3'-quinolone esters, carbamates, and tertiary amines in Escherichia coli. Antimicrob. Agents Chemother. 37(3): 559-565.

    O'Callaghan CH, Sykes RB, Griffiths A, Thornton JE (1976) Cefuroxime, a new cephalosporin antibiotic: Activity in vitro. Antimicrob. Agents Chemother. 9(3):511-519. PMID 1259407

    Salzmann GM (2007) Effects of cefuroxime on human osteoblasts in vitro. J. Biomed. Mat. Res. 82A(2):462-468

    Temel Y, Ayna A, Shafeeq IH and Ciftci M (2018) In vitro effects of some antibiotics on glucose-6-phosphate dehydrogenase from rat (Rattus norvegicus) erythrocyte. Drug Chem. Toxicol. ePub. doi: 10.1080/01480545 PMID 29947262

Other Items In This Category