SKU: N014  / 
    CAS Number: 1476-53-5

    Novobiocin sodium

    $42.11 - $150.31

    Novobiocin sodium is an aminocoumarin antibiotic that prevents cell division. Novobiocin sodium is freely soluble in aqueous solution.

    Mechanism of ActionAminocoumarin antibiotics target DNA gyrase, an enzyme which relieves strain as DNA unwinds during replication and transcription.
    SpectrumNovobiocin sodium targets primarily gram positive organisms and is commonly used to differentiate between coagulase negative (all but S. aureus and a few others) Staphylococcus species. S. saprophyticus is resistant to novobiocin.
    Microbiology ApplicationsNovobiocin sodium is commonly used in clinical in vitro microbiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against gram positive microbial isolates. Medical microbiologists use AST results to recommend antibiotic treatment options for infected patients. Representative MIC values include:

    • Staphylococcus aureus 0.25 µg/mL
    • Staphylococcus saprophyticus 2 µg/mL
    • For a complete list of novobiocin MIC values, click here.
    Plant Biology ApplicationsNovobiocin can be used in combination with gentamicin to prevent and cure Bacillus contamination in plant tissue culture. Benjama et al. demonstrated the potency of this combination in contaminated Date Palm tissue which led to routine use in plant science applications.
    Molecular FormulaC31H35N2NaO11
    References

    Brown, Patrick O., and Craig L. Peebles. "Energy Coupling in DNA Gyrase and the Mechanism of Action of Novobiocin." PNAS 75.2 (1978): 4838-842. Web. 21 Aug. 2012.

    Benjama and Charkaoui, 1997, Control of Bacillus contaminating Date palm tissue in micro- propagation using antibiotics. Pathogen and microbial contamination management in micro- propagation. Kluwer academic publishers.

    MICBifidobacterium adolescentis| ≥15.6|| Bifidobacterium animalis| ≥500|| Bifidobacterium bifidum| ≥500|| Bifidobacterium bifidum | ≥500|| Bifidobacterium breve| ≥7.8|| Bifidobacterium Infantis| ≥500|| Bifidobacterium longum| ≥500|| Bifidobacterium pseudolongum| ≥500|| Bifidobacterium sp.| <0.98 - 15.61|| Bifidobacterium thermophilum| ≥500|| Brucella suis| ≥500|| Enterococcus faecalis| 4 - 12.5|| Enterococcus faecium| 0.4 - 8|| Escherichia coli| 62 - >1024|| Fusobacterium necrophorum| 0.2 - 12.5|| Klebsiella pneumonia| 128 - >512|| Lactobacillus acidophilus| 0.125 - 10|| Lactobacillus brevis| 0.25 - 4|| Lactobacillus bulgaricus| ≥0.98|| Lactobacillus casei| 0.125 - 32|| Lactobacillus curvatus| 4 - 32|| Lactobacillus delbrueckii| 0.125 - 32|| Lactobacillus fermentum| 4 - 16|| Lactobacillus gasseri| ≥10|| Lactobacillus johnsonii| <1|| Lactobacillus lactis| ≥0.98|| Lactobacillus paracasei| 0.98 - <1|| Lactobacillus plantarum| 0.125 - 125|| Lactobacillus rhamnosus| 0.125 - 32|| Lactobacillus sakei| 4 - 32|| Lactobacillus salivarius| <1|| Lactobacillus sp.| 0.125 - 32|| Pseudomonas aeruginosa| ≥32|| Pseudomonas pseudomallei| 1.6 - 12.5|| Salmonella typhimurium| 1.6 - >1024|| Staphylococcus aureus| ≥0.04 - 16|| Staphylococcus auricularis| ≥128|| Staphylococcus capitis| ≥32|| Staphylococcus caprae | ≥64|| Staphylococcus cohnii| 8 - 128|| Staphylococcus epidermidis| <0.04 - 0.25|| Staphylococcus haemolyticus| ≥128|| Staphylococcus hominis| ≥64|| Staphylococcus intermedius| ≥2|| Staphylococcus saprophyticus | ≥2|| Staphylococcus sciuri| ≥8|| Staphylococcus simulans| ≥64|| Staphylococcus warneri| ≥2|| Staphylococcus xylosus| ≥128|| Streptococcus faecium| ≥0.6|| Streptococcus pneumonia| 0.25 - 2|| Streptococcus pyogenes| 0.3 - 2|| Treponema hyodysenteriae | 12.5 - >100|| Xanthomonas maltophilia| >64|| Xylella fastidiosa| 4 - 8||