• Ampicillin/sulbactam (2:1) packaged and labeled.

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SKU: A071

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Description

Ampicillin is a member of the extended spectrum β-lactam family and similar in structure to penicillin. Sulbactam is a β-lactamase inhibitor used to increase potency of β-lactam antibiotics.

TOKU-E offers five forms of ampicillin:

In aqueous solution, ampicillin sodium is freely soluble (50 mg/mL). Ampicillin trihydrate is slightly soluble in water (10 mg/mL) and freely soluble in 1 N HCl (50 mg/mL). Ampicillin anhydrous is sparingly soluble in water and freely soluble in 1 N NH4OH (50 mg/mL). Ampicillin sodium is commonly used to select for successfully transformed bacteria. Ampicillin anhydrous (powder) is the most stable and pure form of ampicillin TOKU-E offers.

    CAS Number

    117060-71-6

    Molecular Formula

    Ampicillin: C16H19N3O4S
    Sulbactam: C8H11NO5S

    Molecular Weight

    Mixture

    Mechanism of Action

    Like all β-lactams, ampicillin interferes with PBP (penicillin binding protein) activity otherwise involved in the final phase of peptidoglycan synthesis. PBP’s are enzymes which catalyze a pentaglycine crosslink between alanine and lysine residues. Without a pentaglycine crosslink, the integrity of the cell wall is severely compromised ultimately leading to cell lysis.

    Tariff Code

    2941.10.5000

    Spectrum

    Ampicillin targets non ESBL (Extended Spectrum β-lactamase) bacteria including Staphylococcus and Streptococcus and medically important enteric pathogens such as Shigella and Salmonella. Interestingly, ampicillin has been found to be effective against certain β-lactam sensitive VRE or vancomycin resistant Enterococcus; a glycopeptide antibiotic resistant "superbug."

Applications

    Eukaryotic Cell Culture Applications

    Ampicillin is routinely used to select for cells containing the pcDNA3.1 and pEAK10 resistance plasmids in cell line A904L at an effective concentration of 50 µg/mL. For additional information on your cell culture needs, please visit our cell-culture database.

    Microbiology Applications

    Ampicillin anhydrous is commonly used in clinical in vitro microbiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against gram positive and gram negative microbial isolates. Medical microbiologists use AST results to recommend antibiotic treatment options for infected patients.

    Media Supplements

    Ampicillin can be used as a selective agent in several types of isolation media:

    Aeromonas Medium Base - Ampicililn Selective Supplement

Specifications

    Form

    Powder

    Appearance

    White or almost white crystalline powder

    Water Content (Karl Fisher)

    ≤2.0%

    pH

    8.0-10.0

    Assay

    Ampicillin: ≥563 μg/mg
    Sulbactam: ≥280 μg/mg

    Impurities

    Dichloromethane: ≤0.06%
    Acetone: ≤0.5%
    Ethanol: ≤0.5%
    Ethyl acetate: ≤0.5%

    Endotoxin

    <0.17 EU/mg

References

    References

    Guzmán, Flavio, MD. "Beta Lactams Antibiotics (penicillins and Cephalosporins) Mechanism of Action.” Medical Pharmacology. Pharmacology Corner, 29 Nov. 2008. Web. 21 Aug. 2012.

    Pitout JD, Sanders CC, Sanders WE Jr. Antimicrobial resistance with focus on beta-lactam resistance in gram-negative bacilli. Am J Med 1997; 103:51.

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