• Puromycin aminonucleoside packaged and labeled.

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SKU: P041

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Description

Puromycin aminonucleoside is a broad spectrum ribosomal chain terminating antibiotic. Puromycin is soluble in aqueous solution.

TOKU-E offers three forms of puromycin: puromycin aminonucleoside (P041), puromycin DiHCl (P001), and puromycin DiHCl solution (P025).  Puromycin aminonucleoside and puromycin DiHCl are soluble in aqueous solution.  Puromycin DiHCl solution is prepared at 10 mg/mL.

    CAS Number

    58-60-6

    Molecular Formula

    C12H18N6O3

    Molecular Weight

    294.31 g/mol

    Mechanism of Action

    Puromycin aminonucleoside is used to study human glomerular disease by inducing nephropathy in laboratory animals.

    Storage Conditions

    2-8 °C

    Tariff Code

    2941.90.1010

Applications

    Eukaryotic Cell Culture Applications

    Puromycin resistant cells express the pac gene which encodes an N-acetyl puromycin transferase.  The pac gene can be mobilized on a plasmid and used to transfect a host cell in an attempt to provide resistance; therefore, puromycin can be used in gene selections for mammalian host cells.  For this application, a kill curve is typically performed to determine the minimum effective puromycin concentration to kill non-resistant cells.  For more information regarding puromycin kill curves, click here.  For additional information regarding relevant cell lines, resistance plasmids, and culture media, please visit our cell culture database

Specifications

    Form

    Powder

    Appearance

    White crystalline

    Source

    Streptomyces Alboniger

    Melting Point

    215-216 °C

References

    References

    Azzam, M. E. "Mechanism of Puromycin Action: Fate of Ribosomes after Release of Nascent Protein Chains from Polysomes." PNAS 70.12 (1973): 3866-3869. www.ncbi.gov. Web. 4 Sept. 2012.

    Vara, J. "Cloning and Expression of a Puromycin N-acetyl Transferase Gene from Streptomyces Alboniger in Streptomyces Lividans and Escherichia Coli." Gene 33.2 (1985): 195-206. Www.ncbi.gov. Web. 7 Sept. 2012.

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