Puromycin Aminonucleoside

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Puromycin Aminonucleoside

SKU: P041

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Description

Puromycin aminonucleoside is a broad spectrum ribosomal chain terminating antibiotic. Puromycin aminonucleoside is soluble in aqueous solution.

TOKU-E offers four forms of puromycin:

Puromycin Aminonucleoside (P041)
Puromycin (P097)
Puromycin Dihydrochloride (P001)
Puromycin Dihydrochloride Solution (P025)

Puromycin has moderate solubility in water while puromycin aminonucleoside and puromycin DiHCl are soluble in aqueous solution. Puromycin DiHCl solution is prepared at 10 mg/mL in 20 mM HEPES buffer.

CAS Number

58-60-6

Molecular Formula

C₁₂H₁₈N₆O₃

Molecular Weight

294.31 g/mol

Mechanism of Action

Puromycin aminonucleoside is used to study human glomerular disease by inducing nephropathy in laboratory animals.

Storage Conditions

2-8 °C

Tariff Code

2941.90.1010

Applications

Eukaryotic Cell Culture Applications

Puromycin resistant cells express the pac gene which encodes an N-acetyl puromycin transferase. The pac gene can be mobilized on a plasmid and used to transfect a host cell in an attempt to provide resistance; therefore, puromycin can be used in gene selections for mammalian host cells. For this application, a kill curve is typically performed to determine the minimum effective puromycin concentration to kill non-resistant cells. For more information regarding puromycin kill curves, click here. For additional information regarding relevant cell lines, resistance plasmids, and culture media, please visit our cell culture database

Specifications

Form

Powder

Appearance

White crystalline

Source

Streptomyces Alboniger

Melting Point

215-216 °C

Technical Data

References

Azzam, M. E. "Mechanism of Puromycin Action: Fate of Ribosomes after Release of Nascent Protein Chains from Polysomes." PNAS 70.12 (1973): 3866-3869. www.ncbi.gov. Web. 4 Sept. 2012.

Vara, J. "Cloning and Expression of a Puromycin N-acetyl Transferase Gene from Streptomyces Alboniger in Streptomyces Lividans and Escherichia Coli." Gene 33.2 (1985): 195-206. Www.ncbi.gov. Web. 7 Sept. 2012.