• Puromycin aminonucleoside packaged and labeled.

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SKU: P041

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Puromycin aminonucleoside is a broad spectrum ribosomal chain terminating antibiotic. Puromycin aminonucleoside is soluble in aqueous solution.

TOKU-E offers four forms of puromycin:

Puromycin has moderate solubility in water while puromycin aminonucleoside and puromycin DiHCl are soluble in aqueous solution. Puromycin DiHCl solution is prepared at 10 mg/mL in 20 mM HEPES buffer.

    CAS Number


    Molecular Formula


    Molecular Weight

    294.31 g/mol

    Mechanism of Action

    Puromycin aminonucleoside is used to study human glomerular disease by inducing nephropathy in laboratory animals.

    Storage Conditions

    2-8 °C

    Tariff Code



    Eukaryotic Cell Culture Applications

    Puromycin resistant cells express the pac gene which encodes an N-acetyl puromycin transferase.  The pac gene can be mobilized on a plasmid and used to transfect a host cell in an attempt to provide resistance; therefore, puromycin can be used in gene selections for mammalian host cells.  For this application, a kill curve is typically performed to determine the minimum effective puromycin concentration to kill non-resistant cells.  For more information regarding puromycin kill curves, click here.  For additional information regarding relevant cell lines, resistance plasmids, and culture media, please visit our cell culture database





    White crystalline


    Streptomyces Alboniger

    Melting Point

    215-216 °C



    Azzam, M. E. "Mechanism of Puromycin Action: Fate of Ribosomes after Release of Nascent Protein Chains from Polysomes." PNAS 70.12 (1973): 3866-3869. www.ncbi.gov. Web. 4 Sept. 2012.

    Vara, J. "Cloning and Expression of a Puromycin N-acetyl Transferase Gene from Streptomyces Alboniger in Streptomyces Lividans and Escherichia Coli." Gene 33.2 (1985): 195-206. Www.ncbi.gov. Web. 7 Sept. 2012.

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