Puromycin Aminonucleoside

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Puromycin Aminonucleoside

SKU: P041

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Description

Puromycin Aminonucleoside is the aminonucleoside portion of Puromycin. It is a Puromycin derivative that lacks the methoxyphenylalanyl group on the amine of the sugar ring. The compound is used in animal models of nephrosis and can induce apoptosis. It has antineoplastic Puromycin was isolated from Streptomyces alboniger in the 1950s. Puromycin is routinely used in cell culture as a selective agent in transfection and transformation protocols to select for cells that have been transformed with the pac gene and express puromycin-N-acetyl-transferase. Puromycin Aminonucleoside is soluble in water.

We also offer:

Puromycin (P097)
Puromycin Dihydrochloride (P001)
Puromycin DiHCl Solution (10 mg/ml in 20 mM HEPES)(P025-P026)

CAS Number

58-60-6

Molecular Formula

C₁₂H₁₈N₆O₃

Molecular Weight

294.31 g/mol

Mechanism of Action

Puromycin Aminonucleoside interferes with protein synthesis, terminating the ribosomal chain.

Storage Conditions

2-8 °C

Tariff Code

2941.90.1010

Spectrum

Gram-positive bacteria, neoplastics, parasites

Applications

Cancer Applications

Puromycin Aminonucleoside is an antimetabolite and antineoplastic agent that is useful in cancer chemotherapy.

Eukaryotic Cell Culture Applications

Cells that are resistant to Puromycin express the pac gene which encodes an N-acetyl puromycin transferase. The pac gene can be mobilized on a plasmid and used to transfect a host cell, thus it can be used in mammalian gene selection protocols. A kill curve is performed to determine the minimum effective concentration to kill non-resistant cells. For additional information regarding cell lines, resistance plasmids, and culture media, please visit our cell culture database.

Puromycin Aminonucleoside can induce apoptosis, thus it can be used to explore the mechanisms of glomerular disease by inducing nephropathy. Glomerular diseases are characterized by podocyte injury or depletion. Podocyte apoptosis is a major factor causing this depletion. Podocytes are specialized epithelial cells in the glomerulus of the kidneys. Nephrin is believed to be the major component of the slit diaphragm, a complex of podocyte-specific proteins that play a role in glomerular filtration. The compound is used to study podocyte biology and quantify the amount of nephrin in a podocyte, to improve our understanding of kidney disease.

Specifications

Form

Powder

Appearance

White crystalline

Source

Streptomyces Alboniger

Melting Point

215-216 °C

Technical Data

References

Azzam ME (1973) Mechanism of Puromycin action: Fate of ribosomes after release of nascent protein chains from polysomes." PNAS 70.12:3866-3869.

Kawakami H et al (2012) Dynamics of absolute amount of nephrin in a single podocyte in Puromycin Aminonucleoside nephrosis rats calculated by quantitative glomerular proteomics approach with selected reaction monitoring mode. Nephrol Dial.Transplant 27(4):1324-1330 PMID 21862459

Lacalle RA, Tercero JA and Jimenez A (1992) Cloning of the complete biosynthetic gene cluster for an aminonucleoside antibiotic, Puromycin, and its regulated expression in heterologous hosts. EMBO J. 11(2):785-792

Vara J (1985) Cloning and expression of a Puromycin N-acetyl transferase gene from Streptomyces alboniger in Streptomyces lividans and Escherichia coli. Gene 33(2):195-206

Wada et al (2005) Dexamethasone prevents podocyte apoptosis induced by Puromycin Aminonucleoside: Role of p53 and Bcl-2-related family proteins. J. Am. Soc. Nephrol. 16(9):2615-2625 PMID 15987750