• Minocycline hydrochloride packaged and labeled.

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SKU: M008

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Description

Minocycline Hydrochloride is the hydrochloride salt of Minocycline, a broad-spectrum tetracycline patented in 1961 by Pfizer and came into commercial use in 1971.  It inhibits bacterial protein synthesis.  It has been found to inhibit PARP1 and nitric oxide synthase.  It is a n NMDA receptor antagonist and has neuroprotective effects.  It also has chondroprotective effects in inflammatory models of arthritis.  Minocycline Hydrochloride is freely soluble in in water.  It is slightly soluble in alcohol.

    CAS Number

    13614-98-7

    Molecular Formula

    C23H27N3O7 ·HCl

    Molecular Weight

    493.94

    Mechanism of Action

    Tetracyclines inhibit bacterial protein synthesis by entering the cell and binding to the 30s ribosomal subunit preventing binding of tRNA. Minocycline acts as a PARP1 inhibitor. It can also indirectly inhibit inducible nitric oxide synthase.

    Storage Conditions

    2-8°C

    Tariff Code

    2941.30.0000

    Spectrum

    Minocycline Hydrochoride has a broad spectrum of activity against Gram-negative and Gram-positive bacteria which cause infections of the upper respiratory tract. It can be used for MRSA.

Applications

    Eukaryotic Cell Culture Applications

    It is a n NMDA receptor antagonist and has neuroprotective effects.  In models of neurodegenerative disease, it has shown promise in disease models of Huntington’s disease and Parkinson’s disease.

    Minocycline has “chondroprotective” effects in inflammatory arthritis animal models.  Nitric oxide (NO) is released from cartilage affected by osteoarthritis (OA) and thus the overproduction of NO has been implicated in the pathogenesis of arthritis.  The effect of Minocycline on the expression of human OA-affected nitric oxide synthase (OA-NOS) and rodent inducible NOS (iNOS).  Minocycline inhibited lipopolysaccharide- and interferon-γ-stimulated iNOS in RAW 264.7 cells in vitro.  It did not influence the specific activity of iNOS in cell-free extracts.  Minocycline should be evaluated as potential therapeutic modulators of NO for various pathological conditions. (Amin et al, 1996).

    Microbiology Applications

    Minocycline Hydrochloride is commonly used in clinical in vitro microbiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against Gram-positive and Gram-negative microbial isolates. Medical microbiologists use AST results to recommend antibiotic treatment options.  Representative MIC values include:

    • Neisseria meningitidis 0.06 µg/mL - 0.12 µg/mL
    • Streptococcus pneumoniae 0.03 µg/mL - 8 µg/mL
    • For a complete list of Minocycline MIC values, click here.

Specifications

    Form

    Powder

    Appearance

    Yellow crystalline powder

    Source

    Synthetic

    Water Content (Karl Fischer)

    4.3-8.0%

    pH

    3.5-4.5

    Assay

    (On Dried Basis): 890 - 950 µg/mg

References

    References

    Amin AR et al (1996)  A novel mechanism of action of tetracyclines: Effects on nitric oxide synthases. Proc. Natl. Acad. Sci. USA 93(24):14014-14019  PMID 8943052

    Chopra I and Marilyn Roberts M (2001)  Tetracycline antibiotics: Mode of action, applications, molecular biology, and epidemiology of bacterial resistance.  Microbiol. and Molec. Biol. Rev. 65(2): 232-260  PMID 11381101

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