• Lincomycin HCl, USP packaged and labeled.

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SKU: L002

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Description

Lincomycin HCl is a lincosamide antibiotic with a very similar mechanism of action to macrolide antibiotics.  Lincomycin HCl is freely soluble in aqueous solution at 29.3 mg/mL.

    CAS Number

    7179-49-9

    Molecular Formula

    C18H34N2O6S · HCl · H2O

    Molecular Weight

    461.02

    Mechanism of Action

    Lincosamide antibiotics inhibit bacterial growth by targeting the 50S ribosomal subunit preventing peptide bond formation and translocation during protein synthesis. Resistance to lincomycin is commonly attributed to mutations in 50S rRNA preventing lincomycin binding allowing the cell to synthesize proteins free of error.

    Storage Conditions

    2-8 °C

    Tariff Code

    2941.90.5000

    Spectrum

    Lincomycin is a broad spectrum antibiotic commonly used to target streptococcal and staphylococcal infections.

Applications

    Microbiology Applications

    Lincomycin HCl is commonly used in clinical in vitro microbiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against gram positive and gram negative microbial isolates. Medical microbiologists use AST results to recommend antibiotic treatment options for infected patients. Representative MIC values include:

    • Staphylococcus aureus 0.2 µg/mL – 3.2 µg/mL
    • Streptococcus pyogenes 0.04 µg/mL – 0.8 µg/mL
    • For a complete list of lincomycin MIC values, click here.

    Plant Biology Applications

    In botanical applications, lincomycin is frequently used as a selection marker in chloroplast transformation. Lincomycin is typically preferred over other antibiotics for chloroplast transformation because it also inhibits callus formation, greening, and subsequent shoot regeneration. To achieve a homoplastic state during chloroplast transformation, 20 cell cycle divisions are required.

Specifications

    Appearance

    White or off-White Crystalline Powder

    Source

    Streptomyces lincolnensis.

    Impurity Profile

    Limit of Lincomycin B: ≤5.0%

    Water Content (Karl Fischer)

    3.0 - 6.0%

    pH

    3.0 - 5.5

    Optical Rotation

    +135° to +150°

    Assay

    Not less than 790 µg/mg

References

    References

    Lovmar, Martin, and Tanel Tenson. "The Mechanism of Action of Macrolides, Lincosamides and Streptogramin B Reveals the Nascent Peptide Exit Path in the Ribosome."Journal of Molecular Microbiology 330.5 (2003): 1005-014.

    Zhijan L.T., Dhekney S.A. and Gray D.J., 2011, Genetic engineering technologies. Plant tissue culture, development and biotechnology, CRC Press

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