• Imipenem mixture w/cilastatin packaged and labeled.

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SKU: I005

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Description

Imipenem is a broad-spectrum β-lactam antimicrobial, a carbapenem effective against extended spectrum beta-lactamase (ESBL) producing Enterobacteriaceae, a group of pathogenic microbes resistant to many first line β-lactam antibiotics and certain cephalosporins.  It is soluble in aqueous solution.

Cilastatin is a chemical substance which inhibits dehydropeptidase, a human enzyme that degrades Imipenem.

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    CAS Number

    74431-23-5; 82009-34-5

    Molecular Formula

    C12H17N3O4S • H2O and C16H26N2O5S

    Molecular Weight

    317.36 and 358.45

    Mechanism of Action

    β-lactams interfere with PBP (penicillin binding protein) activity involved in the final phase of peptidoglycan synthesis. PBP’s are enzymes which catalyze a pentaglycine crosslink between alanine and lysine residues providing additional strength to the cell wall. Without a pentaglycine crosslink, the integrity of the cell wall is severely compromised and ultimately leads to cell lysis and death. Resistance to β-lactams is commonly due to cells containing plasmid encoded β-lactamases. Like many members of the carbapenem subgroup, imipenem is highly resistant to β-lactamase activity.

    Storage Conditions

    2-8°C

    Tariff Code

    2941.90.5000

    Spectrum

    Imipenem is a broad-spectrum antibiotic targeting a wide range of aerobic and anaerobic Gram-positive and Gram-negative bacteria.

Applications

    Microbiology Applications

    Imipenem is commonly used in clinical in vitromicrobiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against Gram-positive and Gram-negative microbial isolates. Medical microbiologists use AST results to recommend antibiotic treatment options.  Representative MIC values include:

    • Pseudomonas aeruginosa 0.25 µg/mL – 12 µg/mL
    • Bacteroides fragilis 0.008 µg/mL – 1 µg/mL
    • For a representative list of imipenem MIC values, click here.

    Plant Biology Applications

    Imipenem has been shown to be effective against bacteria from the genus Burkholderia, a well described plant pathogen, and was shown to be effective for sour skin (onions), slippery skin (bulbs), and cavity disease (mushrooms)(Sojanova et al, 2007).

Specifications

    Form

    Powder

    pH

    6.5 - 8.5

    Assay

    Imipenem: Not less than 400µg/mg
    Cilastatin: Not less than 400µg/mg
    Imipenem/Cilastatin: 0.95 - 1.05: 1

    Loss on Drying

    Not more than 3.5%

References

    References

    Berg PH, Voit EO and White RL (1996)  A pharmacodynamic model for the action of the antibiotic imipenem on Pseudomonas aeruginosa populaitons in vitro. Bltn. Mathcal. Biol. 58(5):923-938 

    Chen P et al (2014)  Activity of Imipenem against Klebsiella pneumoniae Biofilms in vitro and in vivo. Antimicrob. Agents Chemother. 58(2):1208-1213

    Pitout JD, Sanders CC, Sanders WE (1997)  Antimicrobial resistance with focus on beta-lactam resistance in Gram-negative bacilli. Am J Med 103:51

    Stoyanova M, Pavlina I, Moncheva P and Bogatzevska N (2014)  Biodiversity and incidence of Burkholderia species. Biotechnol. & Biotechnlogic. Equip 21(3):306-310  

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