• Imipenem mixture w/cilastatin packaged and labeled.

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SKU: I005

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Description

Imipenem is a sparingly soluble (0.776 mg/mL) β-lactam antibiotic in the carbapenem subgroup.

TOKU-E offers two forms of imipenem: imipenem (I001) and imipenem w/ cilastatin (I005). Cilastatin is a chemical that inhibits the human enzyme dehydropeptidase; an enzyme capable of degrading imipenem.

    CAS Number

    74431-23-5; 82009-34-5

    Molecular Formula

    C12H17N3O4S • H2O and C16H26N2O5S

    Molecular Weight

    317.36 and 358.45

    Mechanism of Action

    β-lactams interfere with PBP (penicillin binding protein) activity involved in the final phase of peptidoglycan synthesis. PBP’s are enzymes which catalyze a pentaglycine crosslink between alanine and lysine residues providing additional strength to the cell wall. Without a pentaglycine crosslink, the integrity of the cell wall is severely compromised and ultimately leads to cell lysis and death. Resistance to β-lactams is commonly due to cells containing plasmid encoded β-lactamases. Like many members of the carbapenem subgroup, imipenem is highly resistant to β-lactamase activity.

    Storage Conditions

    2-8°C

    Tariff Code

    2941.90.5000

    Spectrum

    Imipenem is a broad spectrum antibiotic targeting a wide range of aerobic and anaerobic gram positive and gram negative bacteria.

Applications

    Microbiology Applications

    Imipenem is commonly used in clinical in vitro microbiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against gram positive and gram negative microbial isolates. Medical microbiologists use AST results to recommend antibiotic treatment options for infected patients. Representative MIC values include:

    • Pseudomonas aeruginosa 0.25 µg/mL – 12 µg/mL
    • Bacteroides fragilis 0.008 µg/mL – 1 µg/mL
    • For a complete list of imipenem MIC values, click here.

    Plant Biology Applications

    Sojanova and co-workers (2007) reviewed the activity of imipenem against the causal agents of sour skin, slippery skin, cavity disease, and others in onion, bulbs, and mushrooms respectively. Microbes responsible for these plant illnesses include members of the Burkholderia genus including Burkholderia gladioli. Imipenem activity has shown to be effective against Burkholderia gladioli in some in vitro applications however, imipenem use in plant tissue culture has not been closely studied.

Specifications

    Form

    Powder

    pH

    6.5 - 8.5

    Assay

    Imipenem: Not less than 400µg/mg
    Cilastatin: Not less than 400µg/mg
    Imipenem/Cilastatin: 0.95 - 1.05: 1

    Loss on Drying

    Not more than 3.5%

References

    References

    Stoyanova M., Pavlina I., Moncheva P and Bogatzevska N., 2007, Biodiversity and incidence of Burkholderia species Biotechnol. & Biotechnol. EQ. 21/2007/3

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