• Doxycycline hyclate packaged and labeled.

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SKU: D006

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Description

Doxycycline hyclate is a semisynthetic tetracycline antibiotic and is freely soluble in aqueous solution (50 mg/mL).

    CAS Number

    24390-14-5

    Molecular Formula

    C22H24N2O8 · HCl · 0.5H2O · 0.5C2H6O

    Molecular Weight

    512.94

    Mechanism of Action

    Tetracyclines pass the cell membrane and bind to the 30S ribosomal subunit interfering with the tRNA/mRNA interaction and ultimately inhibiting protein synthesis.

    Storage Conditions

    2-8°C

    Tariff Code

    2941.30.0000

    Spectrum

    Doxycycline hyclate is a broad spectrum antibiotic primarily targeting bacteria responsible for causing respiratory infections including Mycoplasma pneumoniae and Haemophilus influenzae. Doxycycline has been found to be effective against certain β-lactam resistant strains of VRE or vancomycin resistant Enterococcus; a glycopeptide antibiotic resistant "superbug."

Applications

    Eukaryotic Cell Culture Applications

    Doxycycline hyclate is routinely used to select for cells containing the pcDNA3.1 resistance plasmid in cell line 293 at an effective concentration of 2 µg/mL. For additional information on your cell culture needs, please visit our cell-culture database.

    Microbiology Applications

    Doxycycline hyclate is commonly used in clinical in vitro microbiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against gram positive, gram negative, and certain Mycoplasma species. Medical microbiologists use AST results to recommend antibiotic treatment options for infected patients. Representative MIC values include:

    • Mycoplasma pneumoniae 0.03 µg/mL – 0.25 µg/mL
    • Haemophilus influenzae 0.5 µg/mL – 3.1 µg/mL
    • For a complete list of doxycycline MIC values, click here.

    Rose et al. used doxycycline from TOKU-E in methacrylate-based copolymer films and studied their effects on biofilm formation: "Prevention of Biofilm Formation by Methacrylate-Based Copolymer Films Loaded With Rifampin, Clarithromycin, Doxycycline Aone or in Combination." 

Specifications

    Form

    Powder

    Appearance

    Yellow crystalline powder

    Source

    Streptomyces peucetius

    Water Content (Karl Fisher)

    1.4-2.8%

    pH

    2.0-3.0

    Impurities

    Methacycline: Not more than 2.0%
    6-epidoxycycline: Not more than 2.0%
    Other impurities: Not more than 0.5%

References

    References

    Chopra, Ian, and Marilyn Roberts. "Tetracycline Antibiotics: Mode of Action, Applications, Molecular Biology, and Epidemiology of Bacterial Resistance." Microbiology and Molecular Biology Reviews (2001): 232-60. Http://www.ncbi.nlm.nih.gov. Web. 21 Aug. 2012.

    X. Fu, J. G. Menke, Y. Chen, G. Zhou, K. L. MacNaul, S. D. Wright, C. P. Sparrow and E. G. Lund 2001. 27-hydroxycholesterol is an endogenous ligand for liver X receptor in cholesterol-loaded cells

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