• Doxycycline hyclate packaged and labeled.

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SKU: D006

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Description

Doxycycline hyclate is a broad-spectrum tetracycline targeting bacteria responsible for respiratory infections.   It is a matrix metalloproteinase inhibitor, and an inhibitor of mitochondrial biogenesis.  Doxycycline has been shown have anti-cancer properties. Doxycycline hyclate is soluble in aqueous solution.

We also offer:

  • Doxycycline hydrate (D064)
  • Doxycycline hydrochloride (D065)

    CAS Number

    24390-14-5

    Molecular Formula

    C22H24N2O8 · HCl · 0.5H2O · 0.5C2H6O

    Molecular Weight

    512.94

    Mechanism of Action

    Tetracycline antimicrobials bind to the bacterial 30S ribosomal subunit interfering with tRNA/mRNA interaction, ultimately inhibiting protein synthesis. Tetracyclines can inhibit the MMP enzyme family and inhibit mitochondrial biogenesis.

    Storage Conditions

    2-8┬░C

    Tariff Code

    2941.30.0000

    Spectrum

    Doxycycline hyclate has broad-spectrum activity against Gram-positive and Gram-negative bacteria, and Mycoplasma (ie. M. pneumoniae).  Certain β-lactam resistant strains of vancomycin resistant Enterococcus are also inhibited.

Applications

    Cancer Applications

    Using 12 different human tumor cell lines represetnting 8 different cancer types (DCIS, breast, lung, ovarian, pancreatic, prostate, glioblastoma, melanoma), authors found that Doxyclycline inhibited cancer stem cell propagation across this entire panel of cell lines (Lamb et al, 2015).

    Doxycycline can eradicate cancer stem cells in breast cancer patients in vivo. Authors found a quantitative decease in CD44 and ALDH1 expression, biomarkers of ‘stemness’. This is promising work in using cancer stem cells for cancer prevention, and is an excellent candidate for drug repurposing. (Scatena C et al, 2018).

    Eukaryotic Cell Culture Applications

    Doxycycline hyclate is routinely used for gene selection. For additional information on your cell culture needs, please visit our cell-culture database.

    Doxycycline is a matrix metalloproteinase inhibitor.  Using a series of in vitro assays that mimic steps in vascular remodeling, researchers found Doxyclline increased smooth muscle cell adhesion to the substrate.  It also inhibited collagen gel remodeling.  These properties make it potentially useful in vascular disease like atherosclerosis, restenosis, and transplant arteriopathy (Franco et al, 2006).

     

    Microbiology Applications

    Doxycycline hyclate is commonly used in clinical in vitro microbiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against gram positive, gram negative, and certain Mycoplasmaspecies. Medical microbiologists use AST results to recommend antibiotic treatment options.  Representative MIC values include:

    • Mycoplasma pneumoniae 0.03 µg/mL – 0.25 µg/mL
    • Haemophilus influenzae 0.5 µg/mL – 3.1 µg/mL
    • For a complete list of doxycycline MIC values, click here.

Specifications

    Form

    Powder

    Appearance

    yellow crystalline powder

    Source

    Streptomyces peucetius

    Water Content (Karl Fischer)

    1.4-2.8%

    pH

    2.0-3.0

    Impurities

    Methacycline: Not more than 2.0%
    6-epidoxycycline: Not more than 2.0%
    Other impurities: Not more than 0.5%

References

    References

    Catena C et al (2018)  Doxycycline, an inhibitor of mitochondrial biogenesis, effectively reduces cancer stem cells (CSCs) in early breast cancer patients: A clinical pilot study. Front. Oncol. 8:452  PMID 30364293

    Chopra I, and Marilyn Roberts M (2001)  Tetracycline antibiotics: Mode of action, applications, molecular biology, and epidemiology of bacterial resistance." Microbiol. Molec. Biol. Rev. 232-60. 

    Gossen M et al (1995)  Transcriptional activation by tetracyclines in mammalian cells. Science 268(5218):1766-1769  PMID 7792603 PMID 16651635

    Franco et al (2006)  Doxycycline alters vascular smooth muscle cell adhesion, migration, and reorganization of fibrillar collagen matrices. Am. J. Pathol 16895):1697-1709  

    Lamb R et al (2015)  Antibiotics that target mitochondria effectively eradicate cancer stem cells, across multiple tumor types: treating cancer like an infectious disease. Oncotarget. 6(7):4569-84

    TOKU-E reference:

     Rose et al. used Doxycycline from TOKU-E in methacrylate-based copolymer films and studied their effects on biofilm formation: "Prevention of Biofilm Formation by Methacrylate-Based Copolymer Films Loaded With Rifampin, Clarithromycin, Doxycycline Aone or in Combination." 

     

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