Colistin sulfate, USP

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Colistin sulfate, USP

SKU: C083

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Description

Colistin is a unique sparingly soluble cyclic polypeptide antibiotic known as polymyxin E. Colistin has been found to be effective against carbapenem resistant Enterobacteriaceae or CRE. CRE is a "superbug" which possesses NDM-1 or KPC genes which encode New Delhi Metallo-beta-lacamase or Klebsiella pneumoniae cabapenemase respectively; two enzymes which render nearly all beta-lactam antibiotics useless.

TOKU-E offers two forms of colistin: colistin sulfate (C083) and colistin sodium methanesulfonate (C073). Both forms of colistin are freely soluble in aqueous solution. Colistin sodium methanesulfonate (CMS) is considered an inactive prodrug which means it is inactive until converted into colistin by cellular enzymes. Colistin sulfate (colistin) however, is the end product of CMS and does not require molecular conversion for antimicrobial effects. To see all available colistin products, click here.

This product is considered a dangerous good. Quantities above 1 g may be subject to additional shipping fees. Please contact us for specific questions.

CAS Number

1264-72-8

Molecular Formula

C₅₂H₉₈N₁₆O₁₃ •2.5 H₂SO₄ (Colistin Sulfate A)

Molecular Weight

1155.43 (Colistin Sulfate A)

Mechanism of Action

Colistin has a bactericidal effect on Gram negative bacteria by interacting with and displacing essential ions in the lipopolysaccharide (LPS) outer cell wall leading to increased permeability and eventually lysis of the cell.

Storage Conditions

2-8°C

Tariff Code

2941.90.1050

Spectrum

Colistin sulfate is used primarily against Gram negative bacteria.

Applications

Microbiology Applications

Colistin is commonly used in clinical in vitro microbiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against gram negative microbial isolates. Colistin has also shown high potency against high-resistant superbug strains. Medical microbiologists use AST results to recommend antibiotic treatment options for infected patients. Representative MIC values include:

Shigella spp. 64 µg/mL -128 µg/mL
Haemophilus influenzae 0.4 µg/mL – 0.8 µg/mL
For a complete list of colistin MIC values, click here.

Media Supplement

Colistin is routinely used as a selection agent in several types of isolation media:

Columbia Blood Agar - Campylobacter selective supplement (Butzler)

Columbia Blood Agar - Staphylococcus/Streptococcus selective supplement

Columbia Blood Agar - Streptococcus Selective Supplement

Listeria Selective Agar - Listeria Selective Supplement

Listeria Selective Agar - Modified Listeria Selective Supplement

Plant Biology Applications

In plant tissue culture, colistin has been used in combination with chlortetracycline and gentamicin to eliminate Pseudomonas spp. from Prunus infections. Colistin sulfate has been shown to elict alkaloid accumulation in E. californica. Treatment at 0.1 mg/ml for 4 hours showed a 3 times increase in Jasmonate levels over the control (water).

Specifications

Form

Powder

Appearance

White or almost white powder

Source

Bacillus Polymyxa subsp.

Elemental Analysis

As≤2 ppm

Potency (on a dry basis)

≥19,900 u/mg

Absorbance

A₄₇₀ ≤ 0.4

pH

4.0-7.0

Optical Rotation

-63° to -73° (dried substance)

Loss on Drying

≤7.0%

Sulfated Ash

≤1.0%

Heavy Metals

≤20 ppm

Technical Data

References

Falagas, M. E. "Colistin: The Revival of Polymyxins for the Management of Multidrug-resistant Gram-negative Bacterial Infections." Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America 40.9 (2005): 1333-341. www.ncbi.gov. Web. 10 Sept. 2012.

Leifert C., Ritchie J.Y. and Waites W.M., Contaminants of plant-tissue and cell cultures. World Journal of Microbiology and Biotechnology, Vol. 7, pp. 452-469, 1991.

MJ Mueller, W Brodschelm. "Signaling in the elicitation process is mediated through the octadecanoid pathway leading to jasmonic acid". Proc. Natl. Acad. Sci. USA Vol. 90, pp. 7490-7494, August 1993.