• Ceftizoxime sodium packaged and labeled.

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SKU: C021

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Description

Ceftizoxime sodium is a third generation cephalosporin, similar in antibacterial activity to cefotaxime and moxalactam.  It is not hydrolyzed by common plasmid and chromosomal β‐lactamases.   It is soluble in aqueous solution.

    CAS Number

    68401-82-1

    Molecular Formula

    C13H12N5NaO5S2

    Molecular Weight

    405.38

    Mechanism of Action

    Like β-lactams, cephalosporins interfere with PBP (penicillin binding protein) activity involved in the final phase of peptidoglycan synthesis. PBP’s are enzymes which catalyze a pentaglycine crosslink between alanine and lysine residues providing additional strength to the cell wall. Without a pentaglycine crosslink, the integrity of the cell wall is severely compromised and ultimately leads to cell lysis and death. Resistance to cephalosporins is commonly due to cells containing plasmid encoded β-lactamases; however, ceftizoxime is stable in the presence of these inactivating enzymes.

    Storage Conditions

    -20°C

    Tariff Code

    2941.90.5000

    Spectrum

    Ceftizoxime is a broad spectrum antibiotic targeting a wide variety of Gram-positive and Gram-negative bacteria, both aerobic and anaeobic.

Applications

    Eukaryotic Cell Culture Applications

    The effect of cephalosporins on in vitro growth of lymphocytes were studied using  L 5178y mouse lymphoma cells, Molt-4 cells, and murine splenic lymphocytes. Ceftizoxime had no effect on growth of Molt-4 cells at levels of 0.3 to 50 uM and no effect on L 5178y mouse lymphoma cells at 0.1 and 50 uM. The levels of the three polymerases were studied (DNA polymerase α and β, terminal deoxynucleotidyl transferase (TdT) activity) . DNA polymerase β is the major repair polymerase and its activity remains constant in the different cell stages whereas the DNA-replicating DNA polymerase α activity increases at the beginning of S-phase. The TdT, a marker enzyme for immature lymphocytes in the hematopoietic system, reflects the differentiation state of bone marrow pre-T and pre-B cells. The levels of the three polymerases remained unchanged in Molt-4 cells after incubating them with Ceftizoxime at concentrations from 0.1 to 80 µM (Leyhausen et al, 1984).

    Microbiology Applications

    Ceftizoxime sodium is commonly used in clinical in vitro microbiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against Gram-positive and Gram-negative microbial isolates. Medical microbiologists use AST results to recommend antibiotic treatment options for infected patients. Representative MIC values include:

    • Haemophilus spp. 0.008 µg/mL – 0.25 µg/mL
    • Neisseria spp. 0.004 µg/mL – 0.015 µg/mL
    • For a complete list of ceftizoxime MIC values, click here.

Specifications

    Form

    Powder

    Appearance

    White or pale yellow crystalline powder

    Source

    Synthetic

    Water Content (Karl Fischer)

    ≤8.5%

    pH

    6.0-8.0

    Optical Rotation

    +125° to +145°

    Assay

    (On Dried Basis): 850-995 µg/mg

    Impurities

    Individual Impurity: ≤0.5%
    Total Impurities: ≤1.0%
    Ceftizoxime Polymer: ≤0.1%

References

    References

    Fass RJ (1983) Comparative in vitro activities of third-generation cephalosporins. Arch Intern Med. 143(9):1743–1745 PMID 6615095

    Kamimura T, Matsumoto Y, Shigi Y, Nishida M. (1980) Laboratory evaluation of ceftizoxime, a new parenteral cephalosporin. Arzneimittelforschung. 30(10):1662-1664 PMID 6969078

    Leyhausen G, Seibert G, Maidhof A and Muller WEG (1984) Differential stimulation of lymphocyte cell growth in vitro by cephalosporins. Antimicrob. Agents Chemother. 26(5):752-756 PMID 6517558

    Neu, HC (1984) Ceftizoxime: A Beta‐Lactamase‐Stable, Broad‐Spectrum Cephalosporin. J. Human Pharmacol and Drug Ther. 4:47-58

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