Polymyxin B1 Sulfate, EvoPure® is one of the polypeptide fractions in Polymyxin B Sulfate. The fatty acid group found in Polymyxin B1 Sulfate is 6-methyloctanoic acid (6-MOA); the same group found in Polymyxin B1-I Sulfate. Polymyxin B1 however, contains a L-leucine residue, rather than the L-isoleucine reside found in Polymyxin B1-I sulfate. Results from in vitro studies have shown marginal differences in MIC data when comparing the fractions.
We also offer:
- Polymyxin B Sulfate (P007)
- Polymyxin B1-I Sulfate, EvoPure® (P038)
- Polymyxin B2 Sulfate, EvoPure® (P039)
- Polymyxin B3 Sulfate, EvoPure® (P040)
- Polymyxin B6 Sulfate, EvoPure® (P054)
- Polymyxin E1 Sulfate, EvoPure® (P055)
- Polymyxin E2 Sulfate, EvoPure® (P056)
EvoPure® products are purified single antibiotic fractions, most are >99% pure. Highly pure Polymyxin products can be used to analyze the specifc effects of individual Polymyxin B fractions.
|Mechanism of Action||Polymyxin B targets and alters the permeability of lipopolysaccharide (LPS) of Gram-negative bacteria leading to cell lysis. Polymyxin B only needs to interact with LPS, it is not required to enter the cell.|
|Spectrum||Polymyxin B Sulfate targets the outer membrane of Gram-negative bacteria especially Pseudomonas aeruginosa.|
|Microbiology Applications||Polymyxin B Sulfate is commonly used in clinical in vitro microbiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against Gram-negative microbial isolates. Medical microbiologists use AST results to recommend antibiotic treatment options. Representative MIC values include:
|Plant Biology Applications||Polymyxin was successfully tested to counteract phytopathogenic Gram-negative bacteria growth. Polymyxin reduced growth of different strains of Pseudomonas viridiflava at low concentrations (0.08 µg/ml) whereas Erwinia carotovora growth was inhibited at slightly higher concentrations (0.25 µg/ml) (Selim et al, 2005).|
|Molecular Formula||C56H98N16O13 · xH2SO4 (lot specific)|
Kassamali et al used Polymyxin B1, Polymyxin B2, Polymyxin B3, and Polymyxin B1-I (TOKU-E) to test for synergistic and antagonistic effects against various Gram-negative organisms.
Cao G et al (2008) Development and validation of a reversed-phase high-performance liquid chromatography assay for Polymyxin B in human plasma. J. Antimicrob. Chemother. 62(5):1009-1014 PMID 18765414
Mueller MJ, Brodschelm W, Spannagl E and Zenk MH (1993) Signaling in the elicitation process is mediated through the octadecanoid pathway leading to jasmonic acid. Proc. Natl. Acad. Sci. USA 90(16):7490-7494 PMID 11607420
Newton BA (1956) The properties and mode of action of the Polymyxins. Bacter. Rev. 20(1):14-27 PMID 13303920
Orwa JA et al (2001) Isolation and structural characterization of Polymyxin B components. J CHromatogr A 912(2):369-373 PMID 11330807
Selim S, Negrel J, Govaerts C, Gianinazzi S and Tuinen van D (2005) Isolation and partial characterization of antagonistic peptides produced by Paenibacillus sp. strain B2 isolated from the sorghum Mycorrhizosphere. Appl. Environ. Microbiol. 71(11):6501–6507 PMID 16269674
Tam V et al (2011) In vitro potency of various Polymyxin B components.55.9:4490-491
Zavascki AP, Goldani LA, Li J and Nation RL (2007) Polymyxin B for the treatment of multidrug-resistant pathogens: A critical review. J. Antimicrob. Chemother. 60(6):1206-1215 PMID 17878146
|MIC||P. aeruginosa (ATCC 27853)| 4 |1666| A. baumannii (ATCC BAA 747)|2|1666| K. pneumoniae ATCC 13883|2|1666| P. aeruginosa (9019)|4|1666| A. baumannii (1261)|4|1666| K. pneumoniae (VM9)|2|1666||