SKU: P037  / 
    CAS Number: 4135-11-09

    Polymyxin B1 Sulfate, EvoPure®


    Polymyxin B1 Sulfate, EvoPure® is one of the polypeptide fractions in Polymyxin B Sulfate. The fatty acid group found in Polymyxin B1 Sulfate is 6-methyloctanoic acid (6-MOA); the same group found in Polymyxin B1-I Sulfate. Polymyxin B1 however, contains a L-leucine residue, rather than the L-isoleucine reside found in Polymyxin B1-I sulfate. Results from in vitro studies have shown marginal differences in MIC data when comparing the fractions.

    We also offer:

    • Polymyxin B Sulfate (P007)
    • Polymyxin B1-I Sulfate, EvoPure® (P038)
    • Polymyxin B2 Sulfate, EvoPure® (P039)
    • Polymyxin B3 Sulfate, EvoPure® (P040)
    • Polymyxin B6 Sulfate, EvoPure® (P054)
    • Polymyxin E1 Sulfate, EvoPure® (P055)
    • Polymyxin E2 Sulfate, EvoPure® (P056)

    EvoPure® products are purified single antibiotic fractions, most are >99% pure. Highly pure Polymyxin products can be used to analyze the specifc effects of individual Polymyxin B fractions.

    Mechanism of Action Polymyxin B targets and alters the permeability of lipopolysaccharide (LPS) of Gram-negative bacteria leading to cell lysis. Polymyxin B only needs to interact with LPS, it is not required to enter the cell.
    Spectrum Polymyxin B Sulfate targets the outer membrane of Gram-negative bacteria especially Pseudomonas aeruginosa.
    Microbiology Applications Polymyxin B Sulfate is commonly used in clinical in vitro microbiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against Gram-negative microbial isolates. Medical microbiologists use AST results to recommend antibiotic treatment options.  Representative MIC values include:
    • Pseudomonas aeruginosa 0.25 µg/mL – 1 µg/mL
    • For a representative list of Polymyxin B Sulfate MIC values, click here.
    Plant Biology Applications Polymyxin was successfully tested to counteract phytopathogenic Gram-negative bacteria growth. Polymyxin reduced growth of different strains of Pseudomonas viridiflava at low concentrations (0.08 µg/ml) whereas Erwinia carotovora growth was inhibited at slightly higher concentrations (0.25 µg/ml) (Selim et al, 2005).
    Molecular Formula C56H98N16O13 · xH2SO(lot specific)

    Kassamali et al used Polymyxin B1Polymyxin B2Polymyxin B3, and Polymyxin B1-I (TOKU-E) to test for synergistic and antagonistic effects against various Gram-negative organisms.
    in "Microbiological assessment of Polymyxin B components tested alone and in combination"

    Lim et al used Polymyxin B1, B2, B3, and B1-I (TOKU-E) to study the stability of each compound in saline, dextrose, and saline/dextrose infusion solutions in: "Physicochemical stability study of Polymyxin B in various infusion solutions for administration to critically ill patients." 

    Cao G et al (2008)  Development and validation of a reversed-phase high-performance liquid chromatography assay for Polymyxin B in human plasma. J. Antimicrob. Chemother. 62(5):1009-1014  PMID 18765414

    Mueller MJ, Brodschelm W, Spannagl E and Zenk MH (1993)  Signaling in the elicitation process is mediated through the octadecanoid pathway leading to jasmonic acid.  Proc. Natl. Acad. Sci. USA 90(16):7490-7494  PMID 11607420

    Newton BA (1956)  The properties and mode of action of the Polymyxins. Bacter. Rev.  20(1):14-27  PMID 13303920

    Orwa JA et al (2001)  Isolation and structural characterization of Polymyxin B components. J CHromatogr A 912(2):369-373  PMID 11330807

    Selim S, Negrel J, Govaerts C, Gianinazzi S and Tuinen van D (2005)  Isolation and partial characterization of antagonistic peptides produced by Paenibacillus sp. strain B2 isolated from the sorghum Mycorrhizosphere. Appl. Environ. Microbiol. 71(11):6501–6507  PMID 16269674

    Tam V et al (2011)  In vitro potency of various Polymyxin B components.55.9:4490-491

    Zavascki AP, Goldani LA, Li J and Nation RL (2007)  Polymyxin B for the treatment of multidrug-resistant pathogens: A critical review. J. Antimicrob. Chemother.  60(6):1206-1215  PMID 17878146

    MIC P. aeruginosa (ATCC 27853)| 4 |1666| A. baumannii (ATCC BAA 747)|2|1666| K. pneumoniae ATCC 13883|2|1666| P. aeruginosa (9019)|4|1666| A. baumannii (1261)|4|1666| K. pneumoniae (VM9)|2|1666|