SKU: I011  / 
    CAS Number: 367-93-1

    IPTG (Isopropyl β-D-1-thiogalactopyranoside)

    $38.60 - $175.44

    IPTG is a compound that induces transcription of the lac operon by  mimicking allolactose, a lactose metabolite. IPTG is routinely used as a tool in cloning and gene recombination to confirm successful gene insertion. 

    Mechanism of ActionIPTG induces the lac operon by binding to and releasing the lac repressor allowing transcription of lac genes including beta-galactosidase.
    Microbiology ApplicationsIPTG can be used to confirm successful DNA recombination within the lac operon in cloning experiments. X-gal, a chromogenic substrate which turns a blue color when cleaved by IPTG can be used in the media in which the cells are grown. White colonies indicate a gene insertion within the lac operon because beta-galactosidase was not expressed. Blue colonies are indicative of an intact lac operon which expressed beta-galactosidase and subsequently cleaved X-gal.
    ReferencesChang, Lewis M., and N. C. Horton. "Crystal Structure of the Lactose Operon Repressor and Its Complexes with DNA and Inducer." Europe PubMed Central 271.5253 (1996): 1247-254. Europepmc.org. Web. 4 Dec. 2012.