SKU: F027  / 
    CAS Number: 76639-93-5

    Florfenicol Amine

    $86.36 - $302.23

    Florfenicol Amine is a polar metabolite and degradation product of Florfenicol, specifically formed via hydrolysis of the dichloroacetamide of Florfenicol.  Florfenicol Amine has no antibiotic activity but is an important standard for monitoring animal and environmental residues of Florfenicol. Florfenicol Amine is soluble in ethanol, methanol, DMSO and DMF but has limited water solubility. 

    We also carry:

    • Florfenicol (F002)
    Mechanism of ActionAfter entering a bacterial cell, Florfenicol binds to the 50S ribosomal subunit preventing peptide bond formation. Resistance to Florfenicol may be due to decreased cell permeability or a mutation in the 50S ribosomal subunit. 
    Microbiology Applications

    Residues can be detected via liquid chromatography:

    • high performance liquid chromatography (HPLC)
    • ultra-high performance liquid chromatography (UHPLC)
    • ultra-high performance liquid chromatography-electrospray ionization-tandem mass spectrometry (UHPLC-ESI-MS/MS) (ie food samples; chicken muscle)
    • reversed-phase high-performance liquid chromatography with fluorescence detection (RP-HPLC-FLD)(ie eggs)

    Residues can be detected by gas chromatography:

    • Gas chromatography-negative chemical ionization mass spectrometry (GC-NCI/MS) (ie poultry, porcine muscle)
    • Gas chromatography with electron capture detection (ie shrimp)
    Plant Biology Applications

    Breast cancer resistance protein (BCRP) is one of the most important ATP-binding cassette superfamily transporters. Although it was discovered in cancer cells and could recognize anticancer agents, it is also expressed in other normal tissues of animals such as the apical membrane of enterocytes, hepatocytes and renal tubular cells where they can limit GI absorption.  The US FDA has recommended to include whether a candidate compound is a substrate and/or inhibitor of BCRP during its development for those compounds with a net efflux ratio > 2, since it could be considered as a potential substrate of an efflux pump (Ref 9).  Researchers found that Florfenicol is a substrate of BCRP as indicated by the bidirectional transport assay in Madin-Darby canine kidney (MDCK) and MDCK-chABcg2 cells (Liu et al, 2018).

    Solubilitysoluble in ethanol, methanol, DMF or DMSO. Limited water solubility.
    References

    Alechaga É, Moyano E and Galceran MT (2012)  Ultra-high performance liquid chromatography-tandem mass spectrometry for the analysis of phenicol drugs and Florfenicol-Amine in foods. Analyst.137:2486-2494 PMID 22479695

    Hormazabal V, Steffenak I and Yndestad M et al (1993)  Simultaneous determination of residues of Florfenicol and the metabolite Florfenicol Amine in fish tissues by high-performance liquid chromatography. J. Chromatogr. 616:161-165  PMID 8376488

    Xie et al (2011)  Simultaneous determination of thiamphenicol, florfenicol and Florfenicol Amine in eggs by reversed-phase high-performance liquid chromatography with fluorescence detection. J. Chromatog. B 879(23):2351-2354  PMID 21742564

    Yang F, Lin Z, Riviere JE and Baynes RE (2019) Development and application of a population physiologically based pharmacokinetic model for Florfenicol and its metabolite Florfenicol Amine in cattle. Food Chem. Toxicol. 126:285-294  PMID 30825586