• Novobiocin sodium packaged and labeled.

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SKU: N014

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Description

Novobiocin sodium is an aminocoumarin antibiotic that prevents cell division. Novobiocin sodium is freely soluble in aqueous solution.

    CAS Number

    1476-53-5

    Molecular Formula

    C31H35N2NaO11

    Molecular Weight

    634.61

    Mechanism of Action

    Aminocoumarin antibiotics target DNA gyrase, an enzyme which relieves strain as DNA unwinds during replication and transcription.

    Storage Conditions

    2-8 °C

    Tariff Code

    2941.90.1050

    Spectrum

    Novobiocin sodium targets primarily gram positive organisms and is commonly used to differentiate between coagulase negative (all but S. aureus and a few others) Staphylococcus species. S. saprophyticus is resistant to novobiocin.

Applications

    Microbiology Applications

    Novobiocin sodium is commonly used in clinical in vitro microbiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against gram positive microbial isolates. Medical microbiologists use AST results to recommend antibiotic treatment options for infected patients. Representative MIC values include:

    • Staphylococcus aureus 0.25 µg/mL
    • Staphylococcus saprophyticus 2 µg/mL
    • For a complete list of novobiocin MIC values, click here.

    Plant Biology Applications

    Novobiocin can be used in combination with gentamicin to prevent and cure Bacillus contamination in plant tissue culture. Benjama et al. demonstrated the potency of this combination in contaminated Date Palm tissue which led to routine use in plant science applications.

Specifications

    Form

    Powder

    Appearance

    White or yellow powder

    Source

    Synthetic

    Water Content (Karl Fischer)

    ≥6.0%

    Potency (on a dry basis)

    ≥850µg/mg

    pH

    6.5-8.5

    Melting Point

    215-220°C

References

    References

    Brown, Patrick O., and Craig L. Peebles. "Energy Coupling in DNA Gyrase and the Mechanism of Action of Novobiocin." PNAS 75.2 (1978): 4838-842. Web. 21 Aug. 2012.

    Benjama and Charkaoui, 1997, Control of Bacillus contaminating Date palm tissue in micro- propagation using antibiotics. Pathogen and microbial contamination management in micro- propagation. Kluwer academic publishers.

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