• Methotrexate

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SKU: M027

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Description

Methotrexate is a selective agent for dihydrofolate reductase (DHFR)- transfected cells, and a nucleic acid synthesis inhibitor in protein expression systems, thus can be used as an ancillary material in upstream biomanufacturing applications.     It also has immunosuppressive effects for rheumatoid arthritis research and has anti-cancer properties. Methotrexate is soluble in DMSO but practically insoluble in water.

We also offer:

  • Methotraxate sodium salt (M031)
  • Methotrexate, EvoPure® (M091)

This product is considered a dangerous good. Quantities above 1 g may be subject to additional shipping fees. Please contact us for questions.

    CAS Number

    59-05-2

    Molecular Formula

    C20H22N8O5

    Molecular Weight

    454.44

    Mechanism of Action

    In cancerous cells, methotrexate acts as an allosteric inhibitor of dihydrofolate reductase (DHFR), an enzyme involved in the folic acid metabolic pathway, catalyzing the conversion of dihydrofolate to tetrahydrofolate.  Since tetrahydrofolate is needed for synthesis of purine and thymidine synthesis, it results in the inhibition of DNA and RNA synthesis.

Applications

    Cancer Applications

    Methotrexate acts as a chemotherapeutic agent by inhibiting nucleic acid synthesis in cancer cells. In human glioblastoma U87MG cells (brain tumor), Methotrexate can be used as a carrier for PLGA (poly lactic-co-glycolic acid) nanoparticles (Maleki et al, 2017).

    Eukaryotic Cell Culture Applications

    Methotrexate is commonly used in the dihydrofolate reductase (DHFR) selection system as a selection antibiotic to select for DHFR- deficient CHO cells that have been transfected with DHFR genes. Methotrexate inhibits the activity of DHFR; however, cells that overproduce DHFR can tolerate higher concentrations of methotrexate. In most cases, overproducing DHFR cells produce more recombinant protein than lower DHFR producing cells.

    The mammalian cells (ie CHO) used in biopharma manufacturing may exhibit toxicity to USP-grade product, our Methotrexate, EvoPure® is also available.

References

    References

    Fairbanks, LD et al (1999)  Methotrexate inhibits the first committed step of purine biosynthesis in mitogen-stimulated human T-lymphocytes: A metabolic basis for efficacy in rheumatoid arthritis? Biochem. J 342 (1):143-52

    Maleki H et al (2017)  Methotrexate-loaded PLGA nanoparticles: Preparation, characterization and their cytotoxicity effect on human glioblastoma U87MG cells. Med. Nano. Res. 4(1):020
    Kingston RE, Kaufman RJ, Bebbington CR and Rolfe MR (2002)  Amplification using CHO cell expression vectors. Curr. Protoc. Mol. Biol. Chapter 16:Unit 16.23  PMID 18265304

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