Gene Selection Antibiotics

Principles of genetic transformation and selection antibiotics.

Genetic transformation is a term that refers to altering a bacterial cell to take up exogenous DNA to express a specific gene and synthesize a protein of interest. Many times, the DNA is in the form of a recombinant plasmid which is a small, circular piece of DNA that can replicate independently of chromosomal DNA. Before a cell can take up DNA, the cell needs to competent or able to take up extracellular DNA which can be chemically mediated or induced by electroporation. Transformation is a relatively inefficient process and few competent cells actually take up extracellular DNA. To circumvent this issue, a selectable marker (often an antibiotic resistance gene) is used on plasmids to ensure the cells have been successfully transformed. The transformed and non-transformed cells are then grown in the presence of a selection antibiotic and only cells that express the resistance gene along with the rest of the plasmid DNA can grow.

The following antibiotics are frequently used as selection agents for genetic transformation.

Which of the following antibiotic resistance genes do your transformed cells contain? 

 
 
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Packaged and labeled G418 disulfate.
G418 disulfate, also known as G418 sulfate, is routinely used as a selection antibiotic in cell culture gene selection...
Blasticidin S HCl packaged and labeled in glass bottle.
Blasticidin S HCl is a nucleoside antibiotic derived from Streptomyces griseochromogenes and is routinely used in..
Actinomycin D packaged and labeled.
Actinomycin D is an antineoplastic antibiotic that inhibits cell proliferation by acting as a cytotoxic inducer of apoptosis...
Ampicillin anhydrous packaged and labeled.
Ampicillin anhydrous is a member of the extended spectrum β-lactam family and similar in structure...
Blasticidin S packaged and labeled in glass vial.
Blasticidin S is a nucleoside produced by several species of Streptomyces, first reported...
Bleomycin sulfate, USP packaged and labeled.
Bleomycin sulfate is a water soluble anticancer chemotherapeutic agent commonly used to treat Hodgkin’s lymphoma...
Carbenicillin disodium, Ultrapure packaged and labeled.
Carbenicillin disodium, UltraPure is a carboxypenicillin antibiotic and is routinely used in microbiology and plant biology as a selection...
Chloramphenicol palmitate packaged and labeled in glass vial.
Chloramphenicol palmitate is prepared by acylation of chloramphenicol with palmitic...
Erythromycin Estolate packaged and labeled.
Erythromycin estolate is a macrolide antibiotic with a target spectrum similar to penicillin. TOKU-E offers three forms of erythromycin: erythromycin (E002), erythromycin...
G418 disulfate, EvoPure packaged and labeled.
G418 disulfate, EvoPure® is a high purity (≥99.0%) form of G418 disulfate and is free of impurities present in standard grade G418...
Hygromycin B, EvoPure packaged and labeled.
Hygromycin B, EvoPure® solution (100 mg/mL) is a solution containing high purity (>99.0%) hygromycin B...
Irgasan (Triclosan) packaged and labeled.
Irgasan (or triclosan) is a broad spectrum anti-fungal and antibiotic. Irgasan is slightly soluble in water and freely soluble...
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