Gene Selection Antibiotics

Principles of genetic transformation and selection antibiotics.

Genetic transformation is a term that refers to altering a bacterial cell to take up exogenous DNA to express a specific gene and synthesize a protein of interest. Many times, the DNA is in the form of a recombinant plasmid which is a small, circular piece of DNA that can replicate independently of chromosomal DNA. Before a cell can take up DNA, the cell needs to competent or able to take up extracellular DNA which can be chemically mediated or induced by electroporation. Transformation is a relatively inefficient process and few competent cells actually take up extracellular DNA. To circumvent this issue, a selectable marker (often an antibiotic resistance gene) is used on plasmids to ensure the cells have been successfully transformed. The transformed and non-transformed cells are then grown in the presence of a selection antibiotic and only cells that express the resistance gene along with the rest of the plasmid DNA can grow.

The following antibiotics are frequently used as selection agents for genetic transformation.

Which of the following antibiotic resistance genes do your transformed cells contain? 

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Packaged and labeled G418 disulfate.
G418 disulfate is used routinely as a gene selection agent in cell culture.
Blasticidin S HCl packaged and labeled in glass bottle.
Blasticidin S HCl is a nucleoside antibiotic used as a selection agent.
Puromycin packaged and labeled in glass vial.
Puromycin is an aminonucleoside antibiotic with anti-trypanosomal and antineoplastic properties.  
Actinomycin D packaged and labeled.
Actinomycin D is an antineoplastic antibiotic that inhibits cell proliferation by acting as a cytotoxic inducer of apoptosis...
Ampicillin anhydrous packaged and labeled.
Ampicillin Anhydrous is a β-lactam antibiotic commonly used as a selectable marker.
Blasticidin S packaged and labeled in glass vial.
Blasticidin S is a nucleoside produced by several species of Streptomyces.
Bleomycin sulfate, USP packaged and labeled.
Bleomycin sulfate is a chemotherapeutic agent used in Hodgkin’s lymphoma research.
Chloramphenicol palmitate packaged and labeled in glass vial.
Chloramphenicol Palmitate is a prodrug of Chloramphenicol.
Erythromycin Estolate packaged and labeled.
Erythromycin Estolate, a derivative of Erythromycin, is the lauryl sulfate salt of the proprionic ester of Erythromycin.
G418 disulfate, EvoPure packaged and labeled.

G418 Disulfate, EvoPure® is a highly pure (≥ 99.0%) version of our G418 Disulfate. 

Hygromycin B, EvoPure packaged and labeled.
Hygromycin B, EvoPure® solution (100 mg/mL in PBS Buffer) is a solution containing highly pure (>99.0%) Hygromycin B.
Irgasan (Triclosan) packaged and labeled.
Irgasan (Triclosan) is a broad spectrum antibacterial agent.
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