Cycloheximide is a protein synthesis inhibitor in eukaryotic cells and is commonly used to prevent fungal growth.
TOKU-E offers three forms of cycloheximide: cycloheximide (C001), cycloheximide solution (C084), and cycloheximide ultrapure (C071). Cycloheximide solution is dissolved in DMSO at 100 mg/mL. Cycloheximide ultrapure is ≥98% pure and is free of toxic isomers that can potentially kill wanted fungi in a selection media.
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Kadir et al. used cycloheximide from TOKU-E in culture medium to prevent de-novo translation of DVL proteins in neuroblastoma SH-SY5Y cells. "ALFY-Controlled DVL3 Autophagy Regulates Wnt Signaling, Determining Human Brain Size"
Mechanism of Action
Cycloheximide prevents ribosomal translocation during translation as shown in a yeast model. Effective doses are present at concentrations around 200 µM to inhibit yeast growth.
Cycoloheximide is routinely used as a selection agent in several types of isolation media:
Columbia Blood Agar - Campylobacter selective supplement (Butzler)
Dermasel agar - Selective supplement for dermatophyte fungi
Campylobacter Agar - Campylobacter Selective Supplement (Preston)
Listeria Selective Agar - Listeria Selective Supplement
Listeria Enrichemnt Broth - Listeria Selective Enrichment Supplement
Listeria Enrichment Broth - Modified Listeria Selective Enrichemnt Supplement
STAA Agar - STAA Selective Supplement
Legionella CYE Agar - Legionella GVPC Selective Supplement
Campylobacter Agar - Campylobacter Selective Supplement (Karmali)
Bolton Broth - Bolton Broth Selective Supplement
Plant Biology Applications
Cycloheximide is a frequently used lab reagent to inhibit fungal growth by targeting protein synthesis. In yeast, concentrations of 200 µM have fungicidal effects.
Doyle S.M., Diamond M. and McCabe P.F, 2009, Chloroplast and reactive oxygen species involvement in apoptotic-like programmed cell death in Arabidopsis suspension cultures. Journal of Experimental Botany, Vol. 61, No. 2, pp. 473–482, 2010
Schneider-Poetsch T., Ju J., Eyler D.E., Dang Y., Bhat S., Merrick W.C., Green R. and Shen B., 2009, Inhibition of eukaryotic translation elongation by cycloheximide and lactimidomycin. Nature chemical biology, vol 6, march 2010.
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