Cycloheximide packaged and labeled.

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C0011 g
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  • Description

    Cycloheximide is a protein synthesis inhibitor in eukaryotic cells and is commonly used to prevent fungal growth. 

    TOKU-E offers three forms of cycloheximide: cycloheximide (C001), cycloheximide solution (C084), and cycloheximide ultrapure (C071). Cycloheximide solution is dissolved in DMSO at 100 mg/mL. Cycloheximide ultrapure is ≥98% pure and is free of toxic isomers that can potentially kill wanted fungi in a selection media.

    This product is considered a dangerous good. Quantities above 1 g may be subject to additional shipping fees. Please contact us for specific questions.

    Kadir et al. used cycloheximide from TOKU-E in culture medium to prevent de-novo translation of DVL proteins in neuroblastoma SH-SY5Y cells. "ALFY-Controlled DVL3 Autophagy Regulates Wnt Signaling, Determining Human Brain Size"  

    Mechanism of Action

    Cycloheximide prevents ribosomal translocation during translation as shown in a yeast model. Effective doses are present at concentrations around 200 µM to inhibit yeast growth.

    • Microbiology Applications

      Media Supplements

      Cycoloheximide is routinely used as a selection agent in several types of isolation media:

      Columbia Blood AgarCampylobacter selective supplement (Butzler)

      Dermasel agar - Selective supplement for dermatophyte fungi

      Campylobacter Agar - Campylobacter Selective Supplement (Preston)

      Listeria Selective Agar - Listeria Selective Supplement

      Listeria Enrichemnt Broth - Listeria Selective Enrichment Supplement

      Listeria Enrichment Broth - Modified Listeria Selective Enrichemnt Supplement

      STAA Agar - STAA Selective Supplement

      Legionella CYE Agar - Legionella GVPC Selective Supplement

      Campylobacter Agar - Campylobacter Selective Supplement (Karmali)

      Bolton Broth - Bolton Broth Selective Supplement

    • Plant Biology Applications

      Cycloheximide is a frequently used lab reagent to inhibit fungal growth by targeting protein synthesis. In yeast, concentrations of 200 µM have fungicidal effects.

    • References

      Doyle S.M., Diamond M. and McCabe P.F, 2009, Chloroplast and reactive oxygen species involvement in apoptotic-like programmed cell death in Arabidopsis suspension cultures. Journal of Experimental Botany, Vol. 61, No. 2, pp. 473–482, 2010

      Schneider-Poetsch T., Ju J., Eyler D.E., Dang Y., Bhat S., Merrick W.C., Green R. and Shen B., 2009, Inhibition of eukaryotic translation elongation by cycloheximide and lactimidomycin. Nature chemical biology, vol 6, march 2010.



    Purity Level

    (HPLC): ≥90.0%

    Tariff Code


    Related Documents

    For in vitro research use only. Not suitable for human or animal consumption. For MSDSs not available online, please email a request to

  • CAS Number66-81-9
    Molecular FormulaC15H23NO4
    Molecular Weight281.35
    AppearanceWhite or cream-colored powder
    Purity Level(HPLC): ≥90.0%
    SourceStreptomyces griseus
    Potency (on a dry basis)≥900 u/mg (on dried basis)
    Storage ConditionsAmbient
    Loss on Drying≤ 1.0%