• Nitrocefin packaged and labeled in glass bottle.

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SKU: N005

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Description

Nitrocefin is a chromogenic cephalosporin used to detect β-lactamases produced by β-lactam resistant bacteria. It does not appear to have antimicrobial properties, but has useful diagnostic properties.  It can be used as a reagent in beta-lactamase activity studies.  Nitrocefin is soluble in DMSO.

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    CAS Number

    41906-86-9

    Molecular Formula

    C21H16N4O8S2

    Molecular Weight

    516.51

    Mechanism of Action

    β-lactamases hydrolyze the amide bond between the carbonyl carbon and the nitrogen in the β-lactam ring of Nitrocefin. Intact Nitrocefin is converted to hydrolyzed Nitrocefin under visible light (~ 380-500 nm) and a color change from yellow (max at pH 7.0 = 390 nm) to red (max at pH 7.0 = 486 nm).

    Storage Conditions

    -20°C; Protect from light; Store with inert gas.

    Tariff Code

    2934.99.3900

Applications

    Application

    Nitrocefin is useful for the detection of β-lactamase patterns from bacterial cell extracts by isoelectric focusing. It can also be used in biosensor development and protein switch technology.

    Microbiology Applications

    Nitrocefin is used to detect β-lactamase activity from suspected β-lactam resistant bacteria (see protocol below).  It is commonly used at a working concentration of 0.5 - 1.0 mg/ml.

Specifications

    Form

    Powder

    Appearance

    Orange or yellow powder

    Source

    Synthetic

Technical Data

Nitrocefin.jpg

Example of Nitrocefin color change before and after exposure to β-lactamase.

(A) Concentrated Nitrocefin (10.0 mg/mL) in DMSO before dilution with PBS buffer. (B) Nitrocefin diluted with PBS buffer to working concentration (1.0 mg/mL). The yellow color is indicative of intact, undegraded Nitrocefin. (C) 25 units of β-lactamase added to Nitrocefin (1.0 mg/mL in PBS). The red color is the result of β-lactamase-mediated cleavage of the Nitrocefin. (D) Vortexed mixture of contents shown in  (C).

References

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