• Nitrocefin packaged and labeled in glass bottle.

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SKU: N005

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Description

Nitrocefin is a chromogenic cephalosporin used to detect β-lactamases produced by β-lactam resistant bacteria. Nitrocefin is soluble in DMSO.

This product is considered a dangerous good. Quantities above 1 g may be subject to additional shipping fees. Please contact us for details. 

    CAS Number

    41906-86-9

    Molecular Formula

    C21H16N4O8S2

    Molecular Weight

    516.51

    Mechanism of Action

    β-lactamases hydrolyze the amide bond between the carbonyl carbon and the nitrogen in the β-lactam ring of Nitrocefin.  Intact Nitrocefin is converted to hydrolyzed Nitrocefin under visible light (~ 380-500 nm) and a color change from yellow to red is registered.

    Storage Conditions

    -20°C; Protect from light; Store with inert gas.

    Tariff Code

    2934.99.3900

Applications

    Microbiology Applications

    Nitrocefin is used to detect β-lactamase activity from suspected β-lactam resistant bacteria (see protocol below).  It is commonly used at a working concentration of 1.0 mg/ml.

Specifications

    Form

    Powder

    Appearance

    Orange or yellow powder

    Source

    Synthetic

Technical Data

Nitrocefin.jpg

Example of Nitrocefin color change before and after exposure to β-lactamase.

(A) Concentrated Nitrocefin (10.0 mg/mL) in DMSO before dilution with PBS buffer. (B) Nitrocefin diluted with PBS buffer to working concentration (1.0 mg/mL). The yellow color is indicative of intact, undegraded Nitrocefin. (C) 25 units of β-lactamase added to Nitrocefin (1.0 mg/mL in PBS). The red color is the result of β-lactamase-mediated cleavage of the Nitrocefin. (D) Vortexed mixture of contents shown in  (C).

References

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