• Neomycin B sulfate, EvoPure packaged and labeled.

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SKU: N019

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Description

Neomycin B Sulfate, EvoPure®  is the highly purified sulfate salt of Neomycin B, which is the major component found in Neomycin.  Neomycin B is ~ 90% more active than Neomycin A and ~ 65% more active than Neomycin C, its stereoisomer.  Highly pure Neomycin compounds can be used in cell culture, upstream biopharma, and cancer research.

For more Neomycin products, click here.

Custom manufacturing and testing: We are able to prepare custom Neomycin components for your unique specifications.  Additionally, we offer additional testing including endotoxin content, arsenic content, cell line testing, spectral analysis, and more. For more information, please contact us.

    CAS Number

    4146-30-9

    Molecular Formula

    C23H46N6O13 · xH2SO4 (lot specific)

    Molecular Weight

    614.64 (Free base)

    Mechanism of Action

    Aminoglycosides target the 30S ribosomal subunit resulting in an inability to read mRNA ultimately producing a faulty or nonexistent protein.

    Storage Conditions

    -20°C

    Tariff Code

    2941.90.1010

    Spectrum

    Neomycin is a broad-spectrum antibiotic, however, it is mostly used against Gram-negative bacteria.

Applications

    Eukaryotic Cell Culture Applications

    Neomycin is routinely used to select for cells containing resistance plasmids such as pcDNA3 in common cell lines including AtT-20. For additional information for your cell culture needs, please visit our cell-culture database.

    Microbiology Applications

    Neomycin can be used for gene selection, via exploiting the resistance gene (NPT II) (Aragão, 2009).

    Neomycin is commonly used in clinical in vitro microbiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against Gram-positive and Gram-negative microbial isolates. Medical microbiologists use AST results to recommend antibiotic treatment options. Representative effective ranges include:

      • Pseudomonas aeruginosa 0.5 µg/mL – 64 µg/mL
      • Haemophilus influenzae 1.6 µg/mL – 6.3 µg/mL

                  For a representative list of Neomycin MIC values, click here.

    Neomycin Sulfate can be used for food testing in TSN agar to select for Clostridium perfringens and inhibit growth  of Enterobacteria and Clostridium bifermentans.

    Plant Biology Applications

    Neomycin is commonly used in negative selections for plants which have been successfully been transformed with a plasmid conferring resistance via Agrobacterium mediated transformation.

Specifications

    Form

    Powder

    Appearance

    White Powder

    Source

    Streptomyces Fradiae

    pH

    5.7-7.5

Technical Data

HPLC chromatogram showing single fraction of Neomycin B Sulfate, EvoPure®

Neomycin B Sulfate EvoPure

References

    References

    Aragão FJL and Brasileiro ACM (2009) Positive, negative and marker-free strategies for transgenic plant selection. Braz. J. Plant Physiol., 14(1):1-10

    Dai S et al (2001) Comparative analysis of transgenic rice plants obtained by Agrobacterium-mediated transformation and particle bombardment. Mol. Breeding 7: 25–33

    Davis BD (1987)  Mechanism of bactericidal action of aminoglycosides. Microbiol. Rev.  51(3):341-50

    Robertson JH (1971)  Antimicrobial activity of Neomycin C against Staphylococcus epidermidis.  Appl. Microbiol. 22(6): 1164-1165

    Tsuji K and Robertson JH (1969)  Comparative study of responses to Neomycins B and C by microbiological and gas-liquid chromatographic assay methods. App. Microbiol. 18(3):396-398

    Wright JM and Collier B (1977)  The effects of neomycin upon transmitter release and action. J. Pharmacol. and Exper. Ther. 200(3):576-587

    Yuan L and HongPing W (2006)  Rapid analysis of native Neomycin components on a portable capillary electrophoresis system with potential gradient detection.  Analytic. Bioanalyt. Chemi. 385(8):1575-1579

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