• Hygromycin B, EvoPure packaged and labeled.

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SKU: H015

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Hygromycin B, EvoPure® solution (100 mg/mL) is a solution containing high purity (>99.0%) hygromycin B. Hygromycin B is a unique aminoglycoside antibiotic derived from Streptomyces hygroscopicus and is routinely used as a selective agent in cell culture or microbiology applications to isolate hygromycin B resistant cells after transfection or transformation, respectively.

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    CAS Number


    Molecular Formula


    Molecular Weight

    527.52 g/mol

    Mechanism of Action

    Hygromycin B inhibits protein synthesis by strengthening the interaction of tRNA binding in the ribosomal A-site. Hygromycin B also prevents mRNA and tRNA translocation by an unknown mechanism. These are unique mechanisms for an aminoglycoside antibiotic and they differ from the mode of action neomycin, gentamicin, and G418.

    Storage Conditions

    2-8 °C

    Tariff Code



    Hygromycin B is effective against eukaryotic and prokaryotic cells.


    Eukaryotic Cell Culture Applications

    Hygromycin B is routinely used as a selective agent in mammalian cell culture to isolate hygromycin B resistant cells after transfection. Selectable markers for hygromycin B resistant cells include the hyg or hph resistance genes which express a phosphotransferase that inactivates hygromycin B by phosphorylation. Effective working concentrations range from 100 – 1000 µg/mL.

    For additional information regarding relevant cell lines, resistance plasmids, and culture media, please visit our cell culture database.

    Microbiology Applications

    Hygromycin B can be used as a selection agent to isolate hygromycin b resistant bacteria and fungi.





    Streptomyces Hygroscopicus

    Melting Point

    160-180 °C

Technical Data

HPLC, NMR, FTIR, and MS analysis may be available. For more info, please email [email protected].



    Dai S., Zheng P., Marmey P., Zhang S., Tian W., Chen S., Beachy R.N. and Fauquet C. Comparative analysis of transgenic rice plants obtained by Agrobacterium-mediated transformation and particle bombardment. Molecular Breeding 7: 25–33, 2001. © 2001 Kluwer Academic Publishers.

    Schindler, D. "Studies on the Mode of Action of Hygromycin B, an Inhibitor of Translocation in Eukaryotes." Nucleic Acids and Protein Synthesis 521.2 (1978): 459-69. www.ncbi.gov. Web. 6 Sept. 2012.

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