• Hygromycin B, EvoPure packaged and labeled.

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SKU: H010

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Description

Hygromycin B, EvoPure®is a high purity (>99.0%) form of hygromycin B. Hygromycin B is a unique aminoglycoside antibiotic derived from Streptomyces hygroscopicus and is routinely used as a selective agent in cell culture or microbiology applications to isolate hygromycin B resistant cells after transfection or transformation, respectively.

Custom Manufacturing and Testing: TOKU-E Company is able to perform specific tests beyond standard specifications including endotoxin content, arsenic content, cell line testing, spectral analysis, and more. TOKU-E Company has custom manufacturing capabilities and is able to produce custom grade hygromycin B suitable for use in nearly all cell culture applications including use as an ancillary material for upstream pharmaceutical manufacturing. For more information, please contact us. 

This product is considered a dangerous good. Quantities above 1 g may be subject to additional shipping fees. Please contact us for specific questions.

For more hygromycin B products, click here.

For more information on hygromycin B, EvoPure®click here.

    CAS Number

    31282-04-9

    Molecular Formula

    C20H37N3O13

    Molecular Weight

    527.52

    Mechanism of Action

    Hygromycin B inhibits protein synthesis by strengthening the interaction of tRNA binding in the ribosomal A-site. Hygromycin B also prevents mRNA and tRNA translocation by an unknown mechanism. These are unique mechanisms for an aminoglycoside antibiotic and they differ from the mode of action neomycin, gentamicin, and G418.

    Storage Conditions

    2-8°C

    Tariff Code

    2941.90.1010

    Spectrum

    Hygromycin B is effective against eukaryotic and prokaryotic cells.

Applications

    Eukaryotic Cell Culture Applications

    Hygromycin B is routinely used as a selective agent in mammalian cell culture to isolate hygromycin B resistant cells after transfection. Selectable markers for hygromycin B resistant cells include the hyg or hph resistance genes which express a phosphotransferase that inactivates hygromycin B by phosphorylation. Effective working concentrations range from 100 – 1000 µg/mL.

    For additional information regarding relevant cell lines, resistance plasmids, and culture media, please visit our cell culture database.

    Microbiology Applications

    Hygromycin B can be used as a selection agent to isolate hygromycin b resistant bacteria and fungi.

Specifications

    Form

    Powder

    Appearance

    Off-white to tan powder

    Source

    Streptomyces Hygroscopicus

    Water Content (Karl Fisher)

    ≤15%

    Melting Point

    160-180°C

    Assay

    |Plasmid_DNA_Transfection_Protocol.pdf|Selection_of_Stable_Transfected_Cell_Lines_Protocol.pdf

Technical Data

Spectral Data - Hygromycin B, EvoPure®


HNMR Spectra


      

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Solvent: D2O
Instrument: Mercury 300
Frequency: 300 MHz

 

FTIR Spectra

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Mass Spectra

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Polarity/Scan Type: Positive
Solvent: Water
Solution Concentration: 10 mg/mL
References

    References

    Dai S., Zheng P., Marmey P., Zhang S., Tian W., Chen S., Beachy R.N. and Fauquet C. Comparative analysis of transgenic rice plants obtained by Agrobacterium-mediated transformation and particle bombardment. Molecular Breeding 7: 25–33, 2001. © 2001 Kluwer Academic Publishers.

    Schindler, D. "Studies on the Mode of Action of Hygromycin B, an Inhibitor of Translocation in Eukaryotes." Nucleic Acids and Protein Synthesis 521.2 (1978): 459-69. www.ncbi.gov. Web. 6 Sept. 2012.

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