Hygromycin B, EvoPure® is a highly pure form of Hygromycin B (>99.0% pure). Hygromycin B is a unique aminoglycoside antibiotic derived from Streptomyces hygroscopicus and is routinely used as a selection agent in transfection experiments.
TOKU-E Company is able to perform specific tests beyond standard specifications including endotoxin content, arsenic content, cell line testing, spectral analysis, and more.
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CAS Number
31282-04-9
Molecular Formula
C20H37N3O13
Molecular Weight
527.52
Mechanism of Action
Hygromycin B inhibits protein synthesis by strengthening the interaction of tRNA binding in the ribosomal A-site. Hygromycin B also prevents mRNA and tRNA translocation by an unknown mechanism. These are unique mechanisms for an aminoglycoside antibiotic and they differ from the mode of action of neomycin, gentamicin, and G418.
Mechanism of Resistance
Hygromycin B resistance is conferred by the hph gene isolated from Streptomyces hygroscopicus , a 1467 bp fragment which encodes hygromycin B phosphotransferase (HPh). Cell lines successfully transfected with the hph gene produce hygromycin B phosphotransferase and convert hygromycin B to 7”-O-phosphoryl-hygromycin B by phosphorylating the 4-hydroxyl group on the cyclitol ring of hygromycin B. 7”-O-phosphoryl-hygromycin B lacks antibiotic activity and does not interact with prokaryotic or eukaryotic ribosomes.
Storage Conditions
2-8°C
Tariff Code
2941.90.1010
Spectrum
Hygromycin B is effective against eukaryotic and prokaryotic cells.