• Hygromycin B, EvoPure packaged and labeled.

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SKU: H010

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Hygromycin B, EvoPure®is a high purity (>99.0%) form of hygromycin B. Hygromycin B is a unique aminoglycoside antibiotic derived from Streptomyces hygroscopicus and is routinely used as a selective agent in cell culture or microbiology applications to isolate hygromycin B resistant cells after transfection or transformation, respectively.

Custom Manufacturing and Testing: TOKU-E Company is able to perform specific tests beyond standard specifications including endotoxin content, arsenic content, cell line testing, spectral analysis, and more. TOKU-E Company has custom manufacturing capabilities and is able to produce custom grade hygromycin B suitable for use in nearly all cell culture applications including use as an ancillary material for upstream pharmaceutical manufacturing. For more information, please contact us. 

This product is considered a dangerous good. Quantities above 1 g may be subject to additional shipping fees. Please contact us for specific questions.

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For more information on hygromycin B, EvoPure®click here.

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    Mechanism of Action

    Hygromycin B inhibits protein synthesis by strengthening the interaction of tRNA binding in the ribosomal A-site. Hygromycin B also prevents mRNA and tRNA translocation by an unknown mechanism. These are unique mechanisms for an aminoglycoside antibiotic and they differ from the mode of action neomycin, gentamicin, and G418.

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    Hygromycin B is effective against eukaryotic and prokaryotic cells.


    Eukaryotic Cell Culture Applications

    Hygromycin B is routinely used as a selective agent in mammalian cell culture to isolate hygromycin B resistant cells after transfection. Selectable markers for hygromycin B resistant cells include the hyg or hph resistance genes which express a phosphotransferase that inactivates hygromycin B by phosphorylation. Effective working concentrations range from 100 – 1000 µg/mL.

    For additional information regarding relevant cell lines, resistance plasmids, and culture media, please visit our cell culture database.

    Microbiology Applications

    Hygromycin B can be used as a selection agent to isolate hygromycin b resistant bacteria and fungi.





    Off-white to tan powder


    Streptomyces Hygroscopicus

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Technical Data

Spectral Data - Hygromycin B, EvoPure®

HNMR Spectra


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Solvent: D2O
Instrument: Mercury 300
Frequency: 300 MHz


FTIR Spectra

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Mass Spectra

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Polarity/Scan Type: Positive
Solvent: Water
Solution Concentration: 10 mg/mL


    Dai S., Zheng P., Marmey P., Zhang S., Tian W., Chen S., Beachy R.N. and Fauquet C. Comparative analysis of transgenic rice plants obtained by Agrobacterium-mediated transformation and particle bombardment. Molecular Breeding 7: 25–33, 2001. © 2001 Kluwer Academic Publishers.

    Schindler, D. "Studies on the Mode of Action of Hygromycin B, an Inhibitor of Translocation in Eukaryotes." Nucleic Acids and Protein Synthesis 521.2 (1978): 459-69. www.ncbi.gov. Web. 6 Sept. 2012.

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