• Gentamicin A sulfate, EvoPure packaged and labeled.

click on thumbnail to zoom
SKU: G035

Size  
Quantity:
  Click 'Bulk Order' for 10 or more
Price: make selection(s)
Description

Gentamicin A sulfate, EvoPure® is ≥99.0% Gentamicin A.  Gentamicin A sulfate can be used to study the conversion of Gentamicin A to other congeners as the Gentamicin biosynthetic pathway is an attractive target for genetic engineering.

Gentamicin A is a minor component of Gentamicin, along with B, X2 and others.  The minor components account for 20% of Gentamicin as opposed to the major components (gentamicin C1gentamicin C1a, and gentamicin C2) which account for 80% of Gentamicin.

For more Gentamicin products, click here.

    CAS Number

    13291-74-2

    Molecular Formula

    C18H36N4O10 · xH2SO4 (lot specific)

    Molecular Weight

    468.50 g/mol (Free base)

    Mechanism of Action

    Aminoglycosides target the 30S ribosomal subunit resulting in an inability to read mRNA ultimately producing a faulty or nonexistent protein.

    Storage Conditions

    -20°C

    Tariff Code

    2941.90.1010

    Spectrum

    Gentamicin is a broad-spectrum antibiotic targeting a wide range of Gram-positive and Gram-negative bacteria. Gentamicin has been found to be effective against certain β-lactam sensitive VRE or vancomycin resistant Enterococcus; a glycopeptide antibiotic resistant "superbug."

Applications

    Eukaryotic Cell Culture Applications

    The perspective of a safer, less nephrotoxic gentamicin by using a single component, or using a single component for semisynthesis of a novel derivative, is a very attractive idea, and scientists studying the molecular enzymology of the pathway in M. echinospora, used TOKU-E’s Gentamicin A and Gentamycin X2 to assemble a defined set of enzymes to separate single gentamicin C components into pure fractions (Huang et al, 2015). LLC-PK1 porcine proximal tubule cells were exposed to various congeners or native Gentamicin at a concentration of 1 mg/ml. The C2 component of gentamicin had C2 component of gentamicin had minimal cytotoxicity and actually reduced the toxicity of the other Gentamicin components when present in the native mixture. This non-nephrotoxic C2 congener was found to be just as potent against Gram-positive and multidrug-resistant Gram-negative bacteria as native Gentamicin or any of the individual components (Huang et al, 2015).

    Microbiology Applications

    Gentamicin EvoPure® compounds can be used to study effects of individual gentamicin components on various bacterial strains.

Specifications

    Form

    Powder

    Appearance

    White powder

    Source

    Micromonospora spp.

Technical Data

Spectral Data - Gentamicin A, EvoPure®


HNMR Spectra


      

Click to enlarge

Solvent: cdc13
Instrument: Mercury 300
Frequency: 300 MHz

 

FTIR Spectra

Click to enlarge

 

Mass Spectra

Click to enlarge

Polarity/Scan Type: Positive
Solvent: H2O
Solution Concentration: 10 mg/mL
References

    References

    Davis BD (1987) Mechanism of bactericidal action of aminoglycosides. Microbiol. Rev. 51(3): 341-350 PMD 3312985

    Huang C et al (2015) Delineating the biosynthesis of gentamicin X2, the common precursor of the gentamicin C antibiotic complex. Chem. Biol. 22(2):251-261 PMID 25641167

    Stypulkowska K, Blazewicz A, Fijalek Z, Sarna K.(2010) Determination of gentamicin sulphate composition and related substances in pharmaceutical preparations by LC with charged aerosol detection. Chromatograph. 72(11-12):1225-1229 PMID 21212825

    Vydrin, AF (2003) Component composition of gentamicin sulfate preparations. Pharma. Chem. J 37(8): 448-449

Other Items In This Category