• Gentamicin A sulfate, EvoPure packaged and labeled.

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SKU: G035

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Description

Gentamicin A is one of several different components found in standard grade gentamicin. Gentamicin A, gentamicin B, gentamicin X2, and others are considered “minor components,” have little antimicrobial activity, and account for approximately 20% of standard grade gentamicin. The major components of gentamicin are members of the gentamicin C complex which account for up to 80% of standard grade gentamicin and possess higher antimicrobial properties. The minor gentamicin components (A, B, X2, etc..) contain hydroxy groups in the 3’ and 4’ positions of the purpurosamine (2-amino-hexose) ring. The gentamicin C complex does not have hydroxy groups at these locations. This structural difference is thought to account for the difference in antimicrobial activity between the minor (gentamicins A, B, X2, etc..) and the gentamicin C complex. Gentamicin A sulfate, EvoPure® is ≥99.0% gentamicin A.

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Huang et al. used gentamicin A sulfate, EvoPure® and gentamicin X2 sulfate, EvoPure® to study enzymatic conversion of gentamicin A2 to other gentamicin congeners. "Delineating the Biosynthesis of Gentamicin X2, the Common Precursor of the Gentamicin C Antibiotic Complex."

    CAS Number

    13291-74-2

    Molecular Formula

    C18H36N4O10 · xH2SO4 (lot specific)

    Molecular Weight

    468.50 g/mol (Free base)

    Mechanism of Action

    Aminoglycosides target the 30S ribosomal subunit resulting in an inability to read mRNA ultimately producing a faulty or nonexistent protein.

    Storage Conditions

    -20°C

    Tariff Code

    2941.90.1010

    Spectrum

    Gentamicin is a broad spectrum antibiotic targeting a wide range of Gram positive and Gram negative bacteria. Interestingly, gentamicin has been found to be effective against certain β-lactam sensitive VRE or vancomycin resistant Enterococcus; a glycopeptide antibiotic resistant "superbug."

Applications

    Microbiology Applications

    Gentamicin EvoPure® compounds can be used to study effects of individual gentamicin components on various bacterial strains.

Specifications

    Form

    Powder

    Appearance

    White powder

    Source

    Micromonospora spp.

Technical Data

Spectral Data - Gentamicin A, EvoPure®


HNMR Spectra


      

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Solvent: cdc13
Instrument: Mercury 300
Frequency: 300 MHz

 

FTIR Spectra

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Mass Spectra

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Polarity/Scan Type: Positive
Solvent: H2O
Solution Concentration: 10 mg/mL
References

    References

    Davis, Bernard D. "Mechanism of Bactericidal Action of Aminoglycosides."Microbiological Reviews 51.3 (1987): 341-50.

    Sarna, Katarzyna, and Agata Blazewicz. "Determination of Gentamicin Sulphate Composition and Related Substances in Pharmaceutical Preparations by LC with Charged Aerosol Detection." PubMed (2010)

    Vydrin, A. F. "Component Composition of Gentamicin Sulfate Preparations." Pharmaceutical Chemistry Journal 37.8 (2003): 448-49. Web. 15 Oct. 2013.

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