• Chloramphenicol packaged and labeled.

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SKU: C028

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Description

Chloramphenicol is a bacteriostatic antibiotic that inhibits bacterial protein synthesis.

    CAS Number

    56-75-7

    Molecular Formula

    C11H12Cl2N2O5

    Molecular Weight

    323.13 g/mol

    Mechanism of Action

    After entering a bacterial cell, chloramphenicol binds to the 50S ribosomal subunit preventing peptide bond formation. Resistance to chloramphenicol may be due to decreased cell permeability or a mutation in the 50S ribosomal subunit.

    Storage Conditions

    2-8 °C. Store protected from light

    Tariff Code

    2941.40.0000

    Spectrum

    Chloramphenicol is a broad spectrum antibiotic targeting a wide variety of gram positive and gram negative bacteria especially those which cause meningitis.

Applications

    Microbiology Applications

    Chloramphenicol is routinely used to select for transformed cells that express the chloramphenicol resistance gene, cat from a resistance plasmid at concentrations between 25 - 35 µg/mL.

    Media Supplement

    Chloramphenicol is used as a selective agent in Dermasel agar, a growth media designed for selection of dermatophyte fungi from hair, nails, and skin scrapings.

    ChromogenicCandida Agar - Candida Selective Supplement

    Plant Biology Applications

    Because chloroplasts are derived from Cyanobacteria, plants are susceptible to the effects of chloramphenicol. Chloramphenicol can therefore be used as a gene selection agent for resistant plants containing the cat gene.

Specifications

    Form

    Powder

    Appearance

    White to greyish-white or yellowish-white, needle-like crystals or elongated plate

    Source

    Streptomyces venezuela

    pH

    4.5 - 7.5

    Melting Point

    149°C - 153°C

    Optical Rotation

    +17.0º to +20.0º

References

    References

    Sharma K.K., Bhatnagar-Mathur P. and Thorpe T.A. Genetic transformation technology: status and problems. In Vitro Cell. Dev. Biol.—Plant 41:102–112, March–April 2005

    Li W., Ruf S. and Bock R., Chloramphenicol acetyltransferase as selectable marker for plastid transformation. Plant Mol Biol (2011) 76:443–451

    Mayer, Gene, Dr. "Antibiotics - Protein Synthesis, Nucleic Acid Synthesis, and Metabolism." University of South Carolina School of Medicine. Web. 21 Aug. 2012.

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