• Cefotaxime sodium, USP packaged and labeled.

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SKU: C011

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Description

Cefotaxime sodium is a third generation cephalosporin antibiotic.

TOKU-E offers two forms of cefotaxime: cefotaxime sodium, USP (C011) and cefotaxime sodium, CulturePure® (C106). Both forms have are freely soluble in aqueous solution (50 mg/mL). Ultrapure cefotaxime is >99% pure cefotaxime and is free of E-cefotaxime, an otherwise present but toxic isoform.

    CAS Number

    64485-93-4

    Molecular Formula

    C16H16N5NaO7S2

    Molecular Weight

    447.45

    Mechanism of Action

    Like β-lactams, cephalosporins interfere with PBP (penicillin binding protein) activity involved in the final phase of peptidoglycan synthesis. PBP’s are enzymes which catalyze a pentaglycine crosslink between alanine and lysine residues providing additional strength to the cell wall. Without a pentaglycine crosslink, the integrity of the cell wall is severely compromised and ultimately leads to cell lysis and death. Resistance to cephalosporins is commonly due to cells containing plasmid encoded β-lactamases. Cefotaxime sodium however, is largely resistant to β-lactamases.

    Storage Conditions

    -20°C

    Tariff Code

    2941.90.5000

    Spectrum

    Cefotaxime sodium has broad spectrum activity against a wide variety of gram positive and gram negative bacteria. However, unlike many cephalosporin antibiotics, cefotaxime sodium is not effective against Pseudomonas aeruginosa.

Applications

    Microbiology Applications

    Cefotaxime sodium is commonly used in clinical in vitro microbiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against gram positive and gram negative microbial isolates. Medical microbiologists use AST results to recommend antibiotic treatment options for infected patients. Representative MIC values include:

    • Neisseria gonorrhoeae 0.008 µg/mL – 0.03 µg/mL
    • Streptococcus pneumoniae ≤0.007 µg/mL - 0.25 µg/mL
    • For a complete list of cefotaxime MIC values, click here.

    Plant Biology Applications

    Cefotaxime is often used in Agrobacterium tumefaciens mediated transformations to manipulate bacterial growth. It is also sometimes used in combination with Vancomycin as it has a synergistic effect. Most studies do not describe any negative effects to the plant using cefotaxime however, a study by Silva (2001) found a lower efficiency of transformation when used at concentrations of >500 µg/ml.

Specifications

    Form

    Powder

    Appearance

    White or almost white powder

    Source

    Semi-synthetic (Acremonium sp.)

    Absorbance

    A430≤0.2

    pH

    4.5-6.5

    Optical Rotation

    +58° to +64°

    Assay

    916-964 μg/mg (on dried basis)

    Loss on Drying

    ≤3.0%

    Impurities

    Chromatographic Purity:
    Single Impurity: ≤1.0%
    Total Impurities: ≤3.0%

References

    References

    Georgopapadakou, N. H. "Mechanisms of Action of Cephalosporin 3'-quinolone Esters, Carbamates, and Tertiary Amines in Escherichia Coli." American Society for Microbiology 37.3 (1992): 559-65. Antimicrobial Agents and Chemotherapy. Web. 21 Aug. 2012.

    Mathias, R.J. and Boyd L.A., Cefotaxime stimulates callus growth, embryogenesis and regeneration in hexaploid bread wheat (Triticumaestivum L. em Thell), Plant Sci., Vol. 46, pp. 217-223, 1986.

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