• Cefotaxime sodium, USP packaged and labeled.

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SKU: C011

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Description

Cefotaxime Sodium, USP is broad-spectrum, third-generation cephalosporin.  It interferes with bacterial peptidoglycan synthesis.  Cefotaxime Sodium is freely soluble in aqueous solution. 

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  • Cefotaxime Sodium, USP (C011) 

Cefotaxime Sodium, USP conforms to United States Pharmacopoeia specifications.

    CAS Number

    64485-93-4

    Molecular Formula

    C16H16N5NaO7S2

    Molecular Weight

    447.45

    Mechanism of Action

    Like β-lactams, cephalosporins interfere with PBP (penicillin binding protein) activity involved in the final phase of peptidoglycan synthesis. PBP’s are enzymes which catalyze a pentaglycine crosslink between alanine and lysine residues providing additional strength to the cell wall. Without a pentaglycine crosslink, the integrity of the cell wall is severely compromised and ultimately leads to cell lysis and death. Resistance to cephalosporins is commonly due to cells containing plasmid encoded β-lactamases. Cefotaxime Sodium however, is largely resistant to β-lactamases.

    Storage Conditions

    -20°C

    Tariff Code

    2941.90.5000

    Spectrum

    Cefotaxime Sodium has broad-spectrum activity against a wide variety of Gram-positive and Gram-negative bacteria. However, unlike many cephalosporin antibiotics, cefotaxime sodium is not effective against Pseudomonas aeruginosa.

Applications

    Microbiology Applications

    Cefotaxime Sodium is commonly used in clinical in vitro microbiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against Gram-positive and Gram-negative microbial isolates. Medical microbiologists use AST results to recommend antibiotic treatment options.  Representative MIC values include:

      • Neisseria gonorrhoeae 0.008 µg/mL – 0.03 µg/mL
      • Streptococcus pneumoniae ≤0.007 µg/mL - 0.25 µg/mL
      • For a complete list of Cefotaxime MIC values, click here.

    Plant Biology Applications

    Cefotaxime is often used in Agrobacterium tumefaciens mediated transformations to combat bacterial growth. It is also sometimes used in combination with Vancomycin as it has a synergistic effect.   

Specifications

    Form

    Powder

    Appearance

    White or almost white powder

    Source

    Semi-synthetic (Acremonium sp.)

    Absorbance

    A430≤0.2

    pH

    4.5-6.5

    Optical Rotation

    +58° to +64°

    Assay

    916-964 μg/mg (on dried basis)

    Loss on Drying

    ≤3.0%

    Impurities

    Chromatographic Purity:
    Single Impurity: ≤1.0%
    Total Impurities: ≤3.0%

References

    References

    General:

    Georgopapadakou NH (1992)  Mechanisms of action of cephalosporin 3'-quinolone esters, carbamates, and tertiary amines in Escherichia coli. Antimicrob. Agents. Chemother.  37(3): 559-65

    Mathias RJ and Boyd LA (1986)  Cefotaxime stimulates callus growth, embryogenesis and regeneration in hexaploid bread wheat (Triticum aestivum L. em Thell). Plant Sci. 46:217-223

    Cefotaxime Sodium from TOKU-E:

    Ferrer-González E, Kaul M, Parhi AK, LaVoie EJ and Pilch DS (2017) Lactam antibiotics with a high affinity for PBP2 act synergistically with the FtsZ-targeting agent TXA707 against methicillin-resistant Staphylococcus aureus. Antimicrob. Agents Chemother. 61(9):e00863-17

    Lefurgy ST et al (2016)  Analysis of the structure and function of FOX-4 cephamycinase. Antimicrob. Agents. Chemother. 

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