• Bacitracin A, EvoPure packaged and labeled.
  • Bacitracin A, EvoPure Molecule Structure

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SKU: B015

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Description

Bacitracin A, EvoPure® is a highly purified form of Bacitracin A.  Bacitracin A is the most active and potent Bacitracin fraction.  The EvoPure line of Bacitracins are bioactive, non-toxic congeners that have not shown toxicity to cell lines in eukaryotic cell culture.

Bacitracin A, EvoPure can be used to study properties and characteristics of Bacitracin A separately from other Bacitracin compounds found in  standard grade bacitracin. Bacitracin A, EvoPure can also be used as an analytical standard.

For all Bacitracin products, click here.

    CAS Number

    22601-59-8

    Molecular Formula

    C66H103N17O16S

    Molecular Weight

    1422.71

    Mechanism of Action

    Bacitracin prevents phosphorylation of bactoprenol, a transport protein which carries peptidoglycan components outside the cell membrane. Without the active phosphorylated bactoprenol, peptidoglycan synthesis cannot be completed and the cell lyses. Resistance to Bacitracin is understood to involve two mechanisms: A protein transporter (BcrABC) which pumps bacitracin out of the cell after it has entered, and via another protein (BacA) which provides the active phosphorylated bactoprenol from a different synthetic pathway.

    Storage Conditions

    -20 °C

    Tariff Code

    2941.90.5000

    Spectrum

    Bacitracin primarily targets the cell wall in members of the Gram-positive bacteria including Streptococcus pyogenes and Staphylococcus aureus.

Applications

    Eukaryotic Cell Culture Applications

    Bacitracin can be used for gene selection in cell culture. TOKU-E tested the Selectivity Factor values for the different grades of Bacitracin, and found that range was 41- 350 CulturePure grade versus 2-126 for USP grade, for the cell lines tested (HEK293, NIH/3T3, 293T, CHO-k1, B16). Bacitracin, CulturePure® from TOKU-E was used to inhibit antithymocyte globulin (ATG)-mediated tissue factor (TF) activation and surface-associated, protein disulfide isomerase (PDI) activity in HL60 cells. (Langer et al, 2013) Bacitracin, CulturePure from TOKU-E was used as a PDI inhibitor to study its role in TF activation in cisplatin-treated NSCLC cells. (Jacobsen et al, 2015)

    Microbiology Applications

    Bacitracin is a useful tool to differentiate between ß-hemolytic, group A Streptococci (Streptococcus pyogenes) and ß-hemolytic Streptocococci of other groups. Bacitracin can be used as a supplement in chocolate agar to facilitate the isolation of Haemophilus influenzae. Bacitracin can be used to study the regulatory network in B. subtilis. By systematically analyzing the Bacitracin stimulon, authors can pinpoint the loci induced by Bacitracin (Mascher et al 2003).

    Plant Biology Applications

    Tobacco hairy roots and cell suspensions were used in plant transformation studies to produce full length murine IgG1 monoclonal antibody. Bacitracin has been shown to prevent degradation of peptides and hormones in plant systems. Treatment with Bacitracin was not sufficient to prevent loss of antibody from the cultures, but improved the growth rates by up to 53%. (Sharp and Doran, 1999).

Specifications

    Form

    EvoPure® Powder

    Appearance

    White or off-white powder

    Source

    Bacillus subtilus

    Assay

    ≥95% (HPLC)

Technical Data

Spectral Data - Bacitracin A, EvoPure®


HNMR Spectra


   

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Concentration: 3.6 mg in ca. 0.75 mL D2O
Instrument: AV-500
Frequency: 500 MHz



FTIR Spectra


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Position: 698.8 Intensity: 0.0946
Position: 1242.8 Intensity: 0.0713
Position: 1387.4 Intensity: 0.0951
Position: 1517.7 Intensity: 0.150
Position: 1638.8 Intensity: 0.154
Position: 2960.8 Intensity: 0.0575
Position: 3270.8 Intensity 0.0570
References

    References

    Bell, RG (1992) Preparative high-performance liquid chromatographic separation and isolation of Bacitracin components and their relationship to microbiological activity. J. Chromatog. 590:163-68

    Cain BD, Norton PG, Eubanks W, Nick HS and Allen CM (1993) Amplification of the bacA gene confers bacitracin resistance to Escherichia coli. J. Bacteriol. 175(12):3784-3789 PMID 8389741

    Jacobsen C et al (2015) Regulation of tissue factor in NT2 germ cell tumor cells by cisplatin chemotherapy. Thromb Res. 136(3):673-681 PID 26205155

    Langer F et al (2013) Rapid activation of monocyte tissue factor by antithymocyte globulin is dependent on complement and protein disulfide isomerase. Blood 121 (12):2324-2335 PMID 23315166

    Mascher T, Margulis NG, Wang T, Ye RW, Helmann JD (2003) Cell wall stress responses in Bacillus subtilis: The regulatory network of the bacitracin stimulon. Mol. Microbiol 50(5):1591-1604 PMID 14651641

    Mueller MJ, Brodschelm W (1993) Signaling in the elicitation process is mediated through the octadecanoid pathway leading to jasmonic acid. Proc. Natl. Acad. Sci. 90: 7490-7494 PMID 11607420

    Sharp, JM and Doran, PM.(1999) Effect of bacitracin on growth and monoclonal antibody production by tobacco hairy roots and cell suspensions. Biotechnol. Bioprocess Eng. 4: 253

    Stone KJ and Strominger JL (1971) Mechanism of action of bacitracin: Complexation with metal ion and C55-isoprenyl pyrophosphate. PNAS 68 (12): 3223-3227 PMID 4332017

    Webb, NE “ Dose-response models reveal critical features of inhibitor activity and viral infection.” phD diss. UCLA, 2015

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