• Bacitracin A, EvoPure packaged and labeled.
  • Bacitracin A, EvoPure Molecule Structure

click on thumbnail to zoom
SKU: B015

Size  
Quantity:
  Click 'Bulk Order' for 10 or more
Price: make selection(s)
Description

Bacitracin is comprised of numerous antimicrobial peptide fractions. Notable fractions include A, A1, B, B1, B2, C, D, E, F, G, and X. Standard grade bacitracin (B002) is a mixture of these fractions. Bacitracin A is the most active and potent peptide fraction in bacitracin and is very similar to its bacitracin B counterpart.

For more bacitracin products, including fractions, click here.

Webb et al. used Bacitracin A, EvoPure to study its effect on protein disulfide isomerase (PDI) inhibition. Read more here: Dose-Response Models Reveal Critical Features of Inhibitor Activity and Viral Infection.

    CAS Number

    22601-59-8

    Molecular Formula

    C66H103N17O16S

    Molecular Weight

    1422.71

    Mechanism of Action

    Bacitracin prevents phosphorylation of bactoprenol; a transport protein which carries peptidoglycan components outside the cell membrane. Without the active phosphorylated bactoprenol, peptidoglycan synthesis cannot be complete and the cell lyses. Two mechanisms of bacitracin resistance are understood. One mechanism relies on a protein called a BcrABC transporter which pumps bacitracin out after it has entered the cell. An alternative mechanism is due to another protein called BacA which provides the active phosphorylated bactoprenol from a different synthetic pathway.

    Storage Conditions

    -20 °C

    Tariff Code

    2941.90.5000

    Spectrum

    Bacitracin primarily targets the cell wall in members of the gram positive bacteria including Streptococcus pyogenes and Staphylococcus aureus.

Applications

    Application

    Bacitracin A, EvoPure® can be used to study properties and characteristics of bacitracin A separately from other bacitracin compounds found in standard grade bacitracin. Bacitracin A, EvoPure® can also be used as an analytical standard.

Specifications

    Form

    EvoPure® Powder

    Appearance

    White or off-white powder

    Source

    Bacillus subtilus

    Assay

    ≥95% (HPLC)

Technical Data

Spectral Data - Bacitracin A, EvoPure®


HNMR Spectra


   

Click here to enlarge

Concentration: 3.6 mg in ca. 0.75 mL D2O
Instrument: AV-500
Frequency: 500 MHz



FTIR Spectra


Click here to enlarge

Position: 698.8 Intensity: 0.0946
Position: 1242.8 Intensity: 0.0713
Position: 1387.4 Intensity: 0.0951
Position: 1517.7 Intensity: 0.150
Position: 1638.8 Intensity: 0.154
Position: 2960.8 Intensity: 0.0575
Position: 3270.8 Intensity 0.0570
References

    References

    Stone, K. John, and Jack L. Strominger. "Mechanism of Action of Bacitracin: Complexation with Metal Ion and C55-Isoprenyl Pyrophosphate." PNAS 68.12 (1971): 3223-227. Biological Laboratories, Harvard University. Web. 21 Aug. 2012.

    Bell, Robert G. "Preparative High-Performance Liquid Chromatographic Separation and Isolation of Bacitracin Components and Their Relationship to Microbiological Activity." Journal of Chromatography 590 (1992): 163-68. Web. 21 Aug. 2012.

Other Items In This Category