SKU: A045

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Description

Antibiotic-antimycotic solution is effective against both Gram-positive and Gram-negative bacteria as well as yeasts and other fungi in eukaryotic cell culture. The solution is composed of penicillin (10,000 units/mL), streptomycin (10,000 ug/mL), and amphotericin B (25 ug/mL) in 0.9% NaCl.

Naleway et al. used antibiotic-antimycotic solution from TOKU-E in RPMI 1640 media to grow Primary Human Fibroblasts. "Metabolic Lysosomal Enzyme Probes." 

    CAS Number

    61-33-6; 57-92-1; 1397-89-3

    Molecular Formula

    C16H18N2O4S; C21H39N7O12; C47H73NO17

    Molecular Weight

    334.39; 581.57; 924.08

    Mechanism of Action

    The penicillin works by destroying the cell walls of non-resistant cells by preventing peptidoglycan linkage followed by the triggering of autolysis. Streptomycin disrupts bacterial protein synthesis by binding the ribosomes. Amphotericin B, the anti-fungal element, works by increasing the permeability of the fungal cell membrane by binding to ergosterol .

    Storage Conditions

    -20 °C

    Tariff Code

    3821.00.0000

Applications

    Eukaryotic Cell Culture Applications

    Antibiotic-antimycotic is a plant cell culture test for typical cell lines like HeLa cells and NIH3T3 for growth contaminants and is the most popular cell culture antimycotic and antibacterial solution.

Specifications

    Form

    Solution

    Appearance

    Light yellow solution

    Source

    Mixture

    pH

    6.3-6.9

    Assay

    Amphotericin B: 25-30µg/mL
    Penicillin G Sodium: 10000-12000 units/mL
    Streptomycin Sulfate: 10000-12000 µg/mL

    Endotoxin

    ≤1EU/mL

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