Nitrocefin is a chromogenic cephalosporin used to detect β-lactamases produced by β-lactam resistant bacteria. Nitrocefin is soluble in DMSO.
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|Application||Nitrocefin is useful for the detection of β-lactamase patterns from bacterial cell extracts by isoelectric focusing. It can also be used in biosensor development and protein switch technology.|
|Mechanism of Action||β-lactamases hydrolyze the amide bond between the carbonyl carbon and the nitrogen in the β-lactam ring of Nitrocefin. Intact Nitrocefin is converted to hydrolyzed Nitrocefin under visible light (~ 380-500 nm) and a color change from yellow (max at pH 7.0 = 390 nm) to red (max at pH 7.0 = 486 nm).|
|Microbiology Applications||Nitrocefin is used to detect β-lactamase activity from suspected β-lactam resistant bacteria (see protocol below). It is commonly used at a working concentration of 0.5 - 1.0 mg/ml.|
Nitrocefin from TOKU-E was used to study:
Parr, TR et al (1984) Simple Screening Method for Beta-lactamase-positive and -negative Ampicillin-resistant Haemophilus Influenzae Isolates." J. Clin. Microbiol. 20(1): (131-132