Cefoperazone Free Acid is a semi-synthetic, broad-spectrum third-generation cephalosporin. Patented in 1974, it was marketed by Pfizer under the trade name Cefobid. The compound has activity against Pseudomonas. It has a tetrazolyl moiety that confers β-lactamase resistance. Cefoperazone may be used as an analytical standard for the determination of cefoperazone in raw bovine milk.
Cefoperazone Free Acid is slightly soluble in DMSO and Methanol.
|Mechanism of Action
|Cephalosporins interfere with PBP (penicillin binding protein) activity involved in the final phase of peptidoglycan synthesis. PBP’s are enzymes which catalyze a pentaglycine crosslink between alanine and lysine residues providing additional strength to the cell wall. Without a pentaglycine crosslink, the integrity of the cell wall is severely compromised and ultimately leads to cell lysis and death. Resistance to cephalosporins is commonly due to cells containing plasmid encoded β-lactamases.
|Cefoperazone Free Acid has broad-spectrum activity, including activity against Pseudomonas. It is effective against a wide variety of Gram-positive and Gram-negative bacteria.
|Cefoperazone is commonly used in clinical in vitro microbiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against Gram-positive and Gram-negative microbial isolates. Medical microbiologists use AST results to recommend antibiotic treatment options.
Representative MIC values include:
Haemophilus influenzae: 0.12 - 0.25 µg/ml
For a representative list of Cefoperazone MIC values, click here.
Crosby MA and Gump DW (1982) Activity of Cefoperazone and two beta-lactamase inhibitors, sulbactam and clavulanic acid, against Bacteroides spp. correlated with beta-lactamase production. Antimicrob. Agents Chemother. 22(3):398-405 PMID 6291451
Matsubara N, Minami S, Muraoka T, Saikawa I and Mitsuhashi S (1979) In vitro antibacterial activity of Cefoperazone (T-1551), a new semisynthetic cephalosporin. Antimicrob. Agents Chemother. 16(6):731-735 PMID 316988